Protein Info for GFF1925 in Hydrogenophaga sp. GW460-11-11-14-LB1
Annotation: 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 73% identical to KGSD1_AZOBR: Alpha-ketoglutaric semialdehyde dehydrogenase 1 (araE) from Azospirillum brasilense
KEGG orthology group: K00135, succinate-semialdehyde dehydrogenase (NADP+) [EC: 1.2.1.16] (inferred from 83% identity to pol:Bpro_0086)MetaCyc: 49% identical to 6-oxohexanoate dehydrogenase (Acinetobacter johnsonii)
6-oxohexanoate dehydrogenase. [EC: 1.2.1.63]
Predicted SEED Role
"2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26)" in subsystem L-Arabinose utilization or Xylose utilization (EC 1.2.1.26)
MetaCyc Pathways
- D-galactarate degradation II (3/3 steps found)
- D-glucarate degradation II (3/3 steps found)
- 4-aminobutanoate degradation III (2/2 steps found)
- D-glucuronate degradation II (4/5 steps found)
- D-galacturonate degradation II (3/5 steps found)
- nylon-6 oligomer degradation (4/7 steps found)
- L-lyxonate degradation (1/3 steps found)
- cyclohexanol degradation (2/5 steps found)
- trans-4-hydroxy-L-proline degradation II (1/4 steps found)
- D-arabinose degradation III (2/6 steps found)
- L-arabinose degradation III (2/6 steps found)
- D-xylose degradation III (1/5 steps found)
- D-xylose degradation V (1/5 steps found)
- superpathway of microbial D-galacturonate and D-glucuronate degradation (12/31 steps found)
- superpathway of pentose and pentitol degradation (8/42 steps found)
KEGG Metabolic Maps
- Ascorbate and aldarate metabolism
- Butanoate metabolism
- Caprolactam degradation
- Glutamate metabolism
- Tyrosine metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.2.1.16
Use Curated BLAST to search for 1.2.1.16 or 1.2.1.26 or 1.2.1.63
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (479 amino acids)
>GFF1925 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) (Hydrogenophaga sp. GW460-11-11-14-LB1) MSYPNTQLFIQGQWRDAQAGETLAVFNPATGKEIGRVAHARQADLDLALAAAQKGFETWR DTPAIERAKVMRAAAGLMRERAGAIARLLTQEQGKPLAEAKGEAMAAADILEWFADEGLR VYGRIVPSRGNLAMRQMVLKDPVGPVAAFTPWNFPINQVVRKLAAALATGCSMIVKAPEE TPAAPAELVRAFADAGLPAGVLGLVYGTPSEISSYLIAHPVIRKITFTGSTPVGKQLAAM AGQHMKRVTMELGGHAPVIVAEDADVALAVKTAGGAKFRNAGQVCISPTRFLVHESIAKE FSAALVKHAAGLAVGDGTTEGTQMGPLANPRRVTAMAEFTKDAVERGATLATGGERIGDE GNFWRPTILTDVPLDARVFNDEPFGPMAAVRSFNTLDEAIAEANRLPYGLAGYAFTRSLK NADLLARRVDVGMLWVNMPAMPSAEMPFGGIKDSGYGSEGGPEAMEAYLNSRAVTIMNV