Protein Info for GFF1922 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Ammonium transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 472 signal peptide" amino acids 1 to 34 (34 residues), see Phobius details transmembrane" amino acids 75 to 96 (22 residues), see Phobius details amino acids 108 to 129 (22 residues), see Phobius details amino acids 165 to 186 (22 residues), see Phobius details amino acids 194 to 215 (22 residues), see Phobius details amino acids 228 to 249 (22 residues), see Phobius details amino acids 261 to 282 (22 residues), see Phobius details amino acids 293 to 314 (22 residues), see Phobius details amino acids 325 to 346 (22 residues), see Phobius details amino acids 352 to 369 (18 residues), see Phobius details amino acids 381 to 402 (22 residues), see Phobius details amino acids 420 to 440 (21 residues), see Phobius details TIGR00836: ammonium transporter" amino acids 73 to 470 (398 residues), 439.3 bits, see alignment E=6.6e-136 PF00909: Ammonium_transp" amino acids 75 to 470 (396 residues), 400.1 bits, see alignment E=4.7e-124

Best Hits

Swiss-Prot: 61% identical to AMTB_ECOLI: Ammonia channel (amtB) from Escherichia coli (strain K12)

KEGG orthology group: K03320, ammonium transporter, Amt family (inferred from 80% identity to aaa:Acav_0289)

MetaCyc: 61% identical to ammonium transporter (Escherichia coli K-12 substr. MG1655)
RXN-9615

Predicted SEED Role

"Ammonium transporter" in subsystem Ammonia assimilation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (472 amino acids)

>GFF1922 Ammonium transporter (Hydrogenophaga sp. GW460-11-11-14-LB1)
MKKLIATLFLGMGLAFGGLNAMAQTAAPAEAPAAAAAPAEAAPAAAPAPAEAAPAAAAEA
APAAAAPALDTGDTAWMLTSTMLVILMTIPGLALFYGGLARSKNMLSVLMHVFVIFSLIT
ILWCVYGYSLTFVGEGKFVGGFDKVFLKGITADTLSGLLPTIPEFVFVAFQSTFAAITCA
LIVGSFAERIKFSAVLLFSVIWFTFSYIPMAHMVWGGGLLGADGALDFAGGTVVHINAGI
AGLVGAYVVGKRIGYGKEALTPHSLTLTMVGASLLWVGWFGFNAGSAGAANGVAGLAFIN
TILATGAATLSWIAGEALHKGKGSMLGAASGAVAGLVAVTPAAGFIGPMGSIALGLIAGL
VCLWGVGGLKKMLGADDAFDVFGVHGVGGIVGAILTAVFAASSLGGVKADDYAIGAQLWV
QIKSVLVTIVWSGVVSFITYKIVDMMVGLRVSEEAEREGLDITSHGETAYNR