Protein Info for GFF1919 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Glycolate dehydrogenase (EC 1.1.99.14), iron-sulfur subunit GlcF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 409 PF13183: Fer4_8" amino acids 22 to 89 (68 residues), 46.8 bits, see alignment E=1.6e-15 PF13237: Fer4_10" amino acids 22 to 86 (65 residues), 30.7 bits, see alignment E=1.1e-10 PF12838: Fer4_7" amino acids 24 to 89 (66 residues), 42.3 bits, see alignment E=3.7e-14 PF13534: Fer4_17" amino acids 24 to 90 (67 residues), 42.7 bits, see alignment E=3e-14 PF02754: CCG" amino acids 169 to 253 (85 residues), 37.9 bits, see alignment E=7.1e-13 amino acids 305 to 389 (85 residues), 41.3 bits, see alignment E=6.3e-14

Best Hits

Swiss-Prot: 55% identical to GLCF_ECOLI: Glycolate oxidase iron-sulfur subunit (glcF) from Escherichia coli (strain K12)

KEGG orthology group: K11473, glycolate oxidase iron-sulfur subunit (inferred from 77% identity to vap:Vapar_0224)

MetaCyc: 55% identical to glycolate dehydrogenase, putative iron-sulfur subunit (Escherichia coli K-12 substr. MG1655)
Glycolate dehydrogenase. [EC: 1.1.99.14]; 1.1.99.14 [EC: 1.1.99.14]

Predicted SEED Role

"Glycolate dehydrogenase (EC 1.1.99.14), iron-sulfur subunit GlcF" in subsystem Glycolate, glyoxylate interconversions or Photorespiration (oxidative C2 cycle) (EC 1.1.99.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.99.14

Use Curated BLAST to search for 1.1.99.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (409 amino acids)

>GFF1919 Glycolate dehydrogenase (EC 1.1.99.14), iron-sulfur subunit GlcF (Hydrogenophaga sp. GW460-11-11-14-LB1)
MQTQLSPEYQQRADGRAAEAILRKCVHCGFCTATCPTYQVLGDELDGPRGRIYLMKQVLE
GAEPTRKTQLHLDRCLTCRNCESTCPSGVDYGHLVDIGRQIVDEKVPRPMGEKAVRWLLK
EGLTSPLFAPAMKLGQAVRPLLPGLLKDKVPVPRAAGKLPTRSHARRVLLLAGCVQPAMM
PSIHAATVRVLDAAGIQGVLAPEAGCCGAVKFHLNDQDGGRAQMRANIDAWWPLIESGVE
AIVMNASGCGVTVKEYGHILQDDPAYAAKAARVSELTRDLSELLPALVPVLRPLLKRAPD
VARVVAFHPPCTLQHGQQLKGGVEQHLGALGFTVQVAKTEAHLCCGSAGTYSVLNPDMAH
TLRDRKLGHLRELQPAVIASANIGCITHLQSGTATPVRHWVEVLDEILG