Protein Info for GFF1895 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 57% identical to BAUC_PSEAE: Putative 3-oxopropanoate dehydrogenase (bauC) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 86% identity to hmg:100211799)

MetaCyc: 78% identical to malonate-semialdehyde dehydrogenase (acetylating) (Halomonas sp. HTNK1)
Malonate-semialdehyde dehydrogenase (acetylating). [EC: 1.2.1.18]

Predicted SEED Role

"Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27)" in subsystem Isobutyryl-CoA to Propionyl-CoA Module or Valine degradation (EC 1.2.1.27)

MetaCyc Pathways

β-alanine degradation II (2/2 steps found)
β-alanine degradation I (1/2 steps found)
acrylate degradation I (3/5 steps found)
propanoyl-CoA degradation II (3/5 steps found)
L-valine degradation I (4/8 steps found)
2,4-dinitrotoluene degradation (2/7 steps found)
myo-inositol degradation I (1/7 steps found)
myo-, chiro- and scyllo-inositol degradation (1/10 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.18 or 1.2.1.27

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (505 amino acids)

>GFF1895 Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MATTPHPIDHFIHGAVTAGTSPRRQDVYNPATGAVSGKVALANAGDVNAAVAAAQAAFPA
WADTPPIRRARVMFKFLELVNLHKDDLARLITAEHGKVFTDAQGEVSRGIDIIEFACGIP
QLLKGDFTDQVSTGMDNWTLRQPLGVVAGITPFNFPVMVPMWMFPVAIAAGNCFVLKPSP
IDPSPSLFMAELLRQAGLPDGVFNVVQGDKEAVDALLVHPDVKAVSFVGSTPIANTIYET
GAHHGKRVQALGGAKNHMVVMPDADLDQAVDALIGSAYGSAGERCMAISVAVLVGDVAER
IMPRLIERTQALQVLNGTNLAAEMGPIVTAAAHTRIAGYIEAGAKEGATLLVDGRGFQGR
DAGEGCDHGFWMGGTLFDHVTTDMKIYKEEIFGPVLSCVRVPDFANAVQIINDHEFGNGV
SCFTRDGHVAREFARRIQVGMVGINVPIPVPMAWHGFGGWKKSLFGDMHAYGEEGVRFYT
KQKSIMQRWPESIGKGAEFIMPTAK