Protein Info for GFF1890 in Variovorax sp. SCN45

Annotation: 3-carboxy-cis,cis-muconate cycloisomerase (EC 5.5.1.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02426: 3-carboxy-cis,cis-muconate cycloisomerase
  • amino acids 19 to 357 (339 residues), 356.8 bits, E=5.1e-111
• PF00206: Lyase_1
  • amino acids 26 to 308 (283 residues), 186.4 bits, E=9e-59
• PF10397: ADSL_C
  • amino acids 374 to 451 (78 residues), 76.7 bits, E=1.5e-25

Best Hits

Swiss-Prot: 48% identical to YBN5_SCHPO: Uncharacterized protein C8E4.05c (SPBC8E4.05c) from Schizosaccharomyces pombe (strain 972 / ATCC 24843)

KEGG orthology group: K01857, 3-carboxy-cis,cis-muconate cycloisomerase [EC: 5.5.1.2] (inferred from 92% identity to vei:Veis_4598)

MetaCyc: 47% identical to trans-aconitate decarboxylase (Ustilago maydis)
RXN-19902 [EC: 4.1.1.113]

Predicted SEED Role

"3-carboxy-cis,cis-muconate cycloisomerase (EC 5.5.1.2)" in subsystem Protocatechuate branch of beta-ketoadipate pathway (EC 5.5.1.2)

MetaCyc Pathways

• aromatic compounds degradation via β-ketoadipate (8/9 steps found)
• protocatechuate degradation II (ortho-cleavage pathway) (4/4 steps found)
• toluene degradation III (aerobic) (via p-cresol) (7/11 steps found)
• itaconate biosynthesis II (1/3 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (21/35 steps found)
• superpathway of aerobic toluene degradation (12/30 steps found)

KEGG Metabolic Maps

• Benzoate degradation via hydroxylation

Isozymes

Compare fitness of predicted isozymes for: 5.5.1.2

Use Curated BLAST to search for 4.1.1.113 or 5.5.1.2

Protein Sequence (460 amino acids)

>GFF1890 3-carboxy-cis,cis-muconate cycloisomerase (EC 5.5.1.2) (Variovorax sp. SCN45)
MNTTRTSIPMASTVVDSILFRDAFGTQKMRQLFSDGALVQRYIDAEVALAKAEARVGVIP
AEAAEVIERESKIERIDFDHMREETDIVGYPILPLVHQLVAMCGEAGRYVHWGATTQDIM
DTAVALQVRDALDSIDADIRELRGILAELAKKHRDTPMAGRTHLQQALPVTFGYKVAIWL
AMFDRHQQRIAELRPRVAVVEFAGAAGTLASLGDKGFEVQKAMAEELKLGVPATTWHVAR
DGFAEAVNLLALVTGSLGKIALDIMIMASTEFAEVYEPFVKGRGASSTMPQKRNPISSEL
MLAASKAVRQHAGLMVDAMVQDFERATGPWHAEWIAIPESFILTSGALHQAKFALGGLIV
DEARMKHNLGISRGLIVAEAVMMGMAPHIGRQQAHDVVYDACRTVNEKGGTLAEALAALP
EVTKHFDRATIDRLTDPANYLGLAPQMVDRALALSAEVAH