Protein Info for GFF1885 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Aspartyl-tRNA(Asn) amidotransferase subunit A (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit A (EC 6.3.5.7)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 75% identical to GATA_CUPNH: Glutamyl-tRNA(Gln) amidotransferase subunit A (gatA) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K02433, aspartyl-tRNA(Asn)/glutamyl-tRNA (Gln) amidotransferase subunit A [EC: 6.3.5.6 6.3.5.7] (inferred from 78% identity to pna:Pnap_0171)

Predicted SEED Role

"Aspartyl-tRNA(Asn) amidotransferase subunit A (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit A (EC 6.3.5.7)" (EC 6.3.5.6, EC 6.3.5.7)

MetaCyc Pathways

L-glutamate and L-glutamine biosynthesis (7/7 steps found)
L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
ammonia assimilation cycle III (3/3 steps found)
L-glutamate biosynthesis I (2/2 steps found)
L-glutamine degradation I (1/1 steps found)
L-citrulline biosynthesis (6/8 steps found)
glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
superpathway of L-citrulline metabolism (8/12 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.6, 6.3.5.7

Use Curated BLAST to search for 6.3.5.6 or 6.3.5.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (496 amino acids)

>GFF1885 Aspartyl-tRNA(Asn) amidotransferase subunit A (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit A (EC 6.3.5.7) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MNELHTLGVGELASAIADKKTSSVEAAQHLLARAKQHSALGAYLAVNEDATLAQARAADA
RIAAGERSPLLGVPLAHKDIFVTKDFVTTAGSKMLEAYQSPFDATVVQRLAAQGAVTLGK
LNCDEFAMGGSNENSAFGAVRNPWDTTRVPGGSSGGSAVAVAARLAPAVTGTDTGGSIRQ
PASFCGVTGIKPTYGRCSRYGMVAFASSLDQAGPMARSAADCAVLLSAMAGPDLDRDSTS
LDHPAEDYSVLLGRPREGATAAQPLKGLRIGLPKEFFGEGCAPDVVATVRAALAEYEKLG
ATLVDVSLPRTELSIPVYYIIAPAEASSNLSRFDGVKFGHRAAKYDDLIDMYKKSRSEGF
GPEVQRRIMIGTYVLSHGYYDAYYLQAQKIRRLIAQDFQAAFAQCDLIAGPVAPTVAWKL
GEKSNDPVANYLADIYTLSTSLAGLPGMSVPAGFGAGGLPVGLQLVGNYFQEGPLLQAAH
AFQQATDWHTRAPQGY