Protein Info for GFF1881 in Variovorax sp. SCN45

Annotation: 2-oxoglutarate/2-oxoacid ferredoxin oxidoreductase, gamma subunit (EC 1.2.7.-) / 2-oxoglutarate/2-oxoacid ferredoxin oxidoreductase, alpha subunit (EC 1.2.7.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03710: 2-oxoacid:acceptor oxidoreductase, alpha subunit
  • amino acids 11 to 597 (587 residues), 579 bits, E=5.1e-178
• PF01558: POR
  • amino acids 21 to 186 (166 residues), 106.7 bits, E=1.5e-34
• PF01855: POR_N
  • amino acids 223 to 387 (165 residues), 164.6 bits, E=3.3e-52

Best Hits

KEGG orthology group: K00174, 2-oxoglutarate ferredoxin oxidoreductase subunit alpha [EC: 1.2.7.3] (inferred from 94% identity to vpe:Varpa_2456)

Predicted SEED Role

"2-oxoglutarate oxidoreductase, alpha subunit (EC 1.2.7.3)" (EC 1.2.7.3)

MetaCyc Pathways

• TCA cycle V (2-oxoglutarate synthase) (9/9 steps found)
• TCA cycle VI (Helicobacter) (7/9 steps found)
• TCA cycle VII (acetate-producers) (7/9 steps found)
• reductive TCA cycle I (7/11 steps found)
• incomplete reductive TCA cycle (4/7 steps found)
• reductive TCA cycle II (7/12 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (22/56 steps found)

KEGG Metabolic Maps

• Citrate cycle (TCA cycle)
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 1.2.7.3

Use Curated BLAST to search for 1.2.7.3

Protein Sequence (608 amino acids)

>GFF1881 2-oxoglutarate/2-oxoacid ferredoxin oxidoreductase, gamma subunit (EC 1.2.7.-) / 2-oxoglutarate/2-oxoacid ferredoxin oxidoreductase, alpha subunit (EC 1.2.7.-) (Variovorax sp. SCN45)
MTQKQIEAVNDFVIKFANVNGSGSASANELFAKAILRMGVPVSPRNIFPSNIQGLPTWYE
VRVTEDGHLGRRGGTDMMVAMNPQTWDADVAELEPGGYLFYDSTRPLPPSKFRDDIRVIG
MPLTEICNAVYQDPRQRQLFKNIVYVGALAILLGIEPEVVEKLFGEQYKGKEKLLASNVQ
ALHLGCDFARENLREPVGLKVRRADRVGGRIFVDGNSAAALGCVYGGATVAAWYPITPSS
SVAEAFQKYCTKFRVDPSTGQHRFAIVQAEDELASIGMVVGAGWNGARAFTATSGPGISL
MAEFIGLAYFAEIPITLINVQRGGPSTGMPTRTQQADILACAYASHGDTKHVLLFPEDPH
ECFEHAAAALDLADRLQTPVFLMTDLDIGMNQRLCEPFAWNDSQAYDRGKVMSAEELEAG
RDFGRYKDVDGDGIPWRTLPGTHPTKGSYFTRGTTRDAYARYSERGPDYIYNMERLLKKF
ATAATLVPQPVLRPAARKTALGVIYFGSTSPSMHEALDALEARDIHLDAMRLRAFPFPES
VARFVAQHDKVFVVEQNRDAQMRSLLVNELDADPARLVRVLHFDGTPITARFIVQAIATH
VHPTKEAA