Protein Info for GFF1873 in Sphingobium sp. HT1-2
Annotation: Dihydroorotase (EC 3.5.2.3)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 33% identical to PYRC_LACF3: Dihydroorotase (pyrC) from Lactobacillus fermentum (strain NBRC 3956 / LMG 18251)
KEGG orthology group: K01465, dihydroorotase [EC: 3.5.2.3] (inferred from 86% identity to sjp:SJA_C1-27110)Predicted SEED Role
"Dihydroorotase (EC 3.5.2.3)" in subsystem De Novo Pyrimidine Synthesis (EC 3.5.2.3)
MetaCyc Pathways
- superpathway of histidine, purine, and pyrimidine biosynthesis (42/46 steps found)
- superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (17/18 steps found)
- UMP biosynthesis II (6/6 steps found)
- superpathway of pyrimidine ribonucleotides de novo biosynthesis (8/9 steps found)
- UMP biosynthesis I (5/6 steps found)
- UMP biosynthesis III (5/6 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.5.2.3
Use Curated BLAST to search for 3.5.2.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (407 amino acids)
>GFF1873 Dihydroorotase (EC 3.5.2.3) (Sphingobium sp. HT1-2) MSRIAIVNGKLADPAGDALVPGTVLIEGDRILATGAVDIPADAQQVDAGGLVVAPGLIDL GVFATDKPAFHFGGITRAALMPDQRAPLDEVGLIREATRAGKPDFWVHPIAAATRGLKGT EMAEMGLMQIAGAKAVGTGRQWIADSGVMLRILSYASGLGLTVIAHAEDGGLSAKAVATS GETATRLGLAHAPACAEAMAIARDIMLARETGAAIHFRLVTTKAGFDLIRAAKAEGLKVS CGISPAYLFLNDQAMTDFRTFARLSPPLRSDADRHASIAAVADGTVDVITSGHDPRGPED KRLPFADASPGMAGAETLLALSLNLVREGHVSLNRLMTLLSANPAKILGVNAGSFAPGSA ADLILVDPDAPWIVDSAKMAASAGNTPFDKVPVQGRARRIMKGGQFF