Protein Info for GFF1840 in Variovorax sp. SCN45

Annotation: Xanthine dehydrogenase iron-sulfur subunit (EC 1.17.1.4) / Xanthine dehydrogenase, FAD binding subunit (EC 1.17.1.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02963: xanthine dehydrogenase, small subunit
  • amino acids 11 to 510 (500 residues), 668.7 bits, E=2.1e-205
• PF00111: Fer2
  • amino acids 17 to 70 (54 residues), 22.6 bits, 1.7e-08
• PF01799: Fer2_2
  • amino acids 123 to 192 (70 residues), 93 bits, E=2e-30
• PF00941: FAD_binding_5
  • amino acids 236 to 399 (164 residues), 165.7 bits, E=1.7e-52
• PF03450: CO_deh_flav_C
  • amino acids 407 to 509 (103 residues), 111.8 bits, E=3.3e-36

Best Hits

KEGG orthology group: K13481, xanthine dehydrogenase small subunit [EC: 1.17.1.4] (inferred from 87% identity to vpe:Varpa_4514)

Predicted SEED Role

"Xanthine dehydrogenase, iron-sulfur cluster and FAD-binding subunit A (1.17.1.4)" in subsystem Purine Utilization

MetaCyc Pathways

• adenosine nucleotides degradation II (5/5 steps found)
• purine nucleotides degradation I (plants) (10/12 steps found)
• adenosine nucleotides degradation I (7/8 steps found)
• guanosine nucleotides degradation II (4/4 steps found)
• purine nucleotides degradation II (aerobic) (9/11 steps found)
• superpathway of guanosine nucleotides degradation (plants) (5/6 steps found)
• guanosine nucleotides degradation I (3/4 steps found)
• guanosine nucleotides degradation III (3/4 steps found)
• inosine 5'-phosphate degradation (3/4 steps found)
• ureide biosynthesis (5/7 steps found)
• superpathway of purines degradation in plants (12/18 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• caffeine degradation III (bacteria, via demethylation) (2/7 steps found)
• caffeine degradation IV (bacteria, via demethylation and oxidation) (2/10 steps found)
• theophylline degradation (1/9 steps found)

KEGG Metabolic Maps

• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.17.1.4

Use Curated BLAST to search for 1.17.1.4

Protein Sequence (548 amino acids)

>GFF1840 Xanthine dehydrogenase iron-sulfur subunit (EC 1.17.1.4) / Xanthine dehydrogenase, FAD binding subunit (EC 1.17.1.4) (Variovorax sp. SCN45)
MSTDSSKIQPIRFFHRGKIVDVTGVHPTRSVLDWLREDARCTGTKEGCNEGDCGACTVVI
GELAASPDAPGAVDGLSLQTVNACIQFLPTLHGKALFTVEDLKAQCQSKPHKTEQKAKDG
KHAVHTLHPVQQAMVDCHGSQCGFCTPGFVMSLWATYEHHQSEGTQPTRQQLADDLSGNL
CRCTGYRPILDAGQRMFDLPSVRLDTRPVVAALNALRDDGAALSYAAPLGARMDHFHAPK
TLTQLATLREQKPRAQLLAGSTDVGLWVNKQFRDLGDIIYVGDVAEMRTIEERKEELYIG
AGASLESAFAALVERVPSLAEVWLRFASPPIRHAGTMGGNVANGSPIGDSPPVLMSLDAQ
IELRRGAAVRRMPLPDFYLDYMKNQLQPGEFVQGLAIPLAAMRRQVRAYKISKRFDCDIS
ALCAGFAIELEEGGDTVKAVRLAFGGMAATVRRAANAEAALVGKPWTQSSVSNAKLALAQ
DFKPLSDMRASADYRLLVAQNLIQRLWLETRAEDALPLEETSVWSVMPHAPATAGGAVGQ
AAKAAEGV