Protein Info for GFF1835 in Variovorax sp. SCN45

Annotation: Indolepyruvate oxidoreductase subunit IorB II (EC 1.2.7.8)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 538 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details transmembrane" amino acids 390 to 409 (20 residues), see Phobius details PF01558: POR" amino acids 15 to 188 (174 residues), 36.9 bits, see alignment E=4.5e-13 PF20169: DUF6537" amino acids 256 to 474 (219 residues), 140.1 bits, see alignment E=9.5e-45

Best Hits

KEGG orthology group: None (inferred from 88% identity to vpe:Varpa_4524)

Predicted SEED Role

"Indolepyruvate oxidoreductase subunit IorB II (EC 1.2.7.8)" in subsystem Aromatic amino acid interconversions with aryl acids (EC 1.2.7.8)

Isozymes

Compare fitness of predicted isozymes for: 1.2.7.8

Use Curated BLAST to search for 1.2.7.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (538 amino acids)

>GFF1835 Indolepyruvate oxidoreductase subunit IorB II (EC 1.2.7.8) (Variovorax sp. SCN45)
MEQNRPLTLLVCALGGEGGGVLTEWLVDIARHAGYAAQSTSIPGVAQRTGATTYYIEVFP
VPLARLGGRRPVFSLSPVPGALDAIVSSELLETARQIGNGMSAPLRTLVISSSARILTTA
ERMEPGDGRADAQRLLDVVKAFSREHHVFDMNAIARDSGTVVSAVMLGAIAGSGLFPFPR
EAYEHVVRGGDTSAPDKLSKMAAASLRGFAAAFDAVSAPRAQAAFVSSVLASDTSDAPPP
AGALPAELARRFPPAVHDMLTLGHARVLDYQDTAYAALYADRLGRVLDAERAADPAGAQG
FAITREMARWLALWMAFDDIVRVAALKGRASRAQRVRQEVRAGEDDIVKVYDHFKPGAAE
FAALLPASLSRRVTAWDRARQARGQEPWALPLKVGSHSVFGMASLRLLASLRWLRRRGSR
FAEEQALIERWLAAVETGTREAWALGHELALCGRLIKGYGSTNERGKHNLLHVIDALAGR
TALPASARTTAIAAAREAALADEGGKALDAALAAHGAAPRPVQAQPIRWMKRPPPLKV