Protein Info for GFF1827 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (EC 1.2.1.60)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 490 TIGR02299: 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase" amino acids 4 to 489 (486 residues), 799.9 bits, see alignment E=3.7e-245 PF00171: Aldedh" amino acids 17 to 476 (460 residues), 577.9 bits, see alignment E=6.7e-178

Best Hits

Swiss-Prot: 66% identical to HPCC_ECOLX: 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (hpcC) from Escherichia coli

KEGG orthology group: K00151, 5-carboxymethyl-2-hydroxymuconic-semialdehyde dehydrogenase [EC: 1.2.1.60] (inferred from 89% identity to bbr:BB0736)

MetaCyc: 67% identical to subunit of 5-carboxymethyl-2-hydroxymuconic semialdehyde dehydrogenase (Escherichia coli B)
5-carboxymethyl-2-hydroxymuconic-semialdehyde dehydrogenase. [EC: 1.2.1.60]

Predicted SEED Role

"5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (EC 1.2.1.60)" in subsystem 4-Hydroxyphenylacetic acid catabolic pathway or Aromatic amino acid degradation or Central meta-cleavage pathway of aromatic compound degradation (EC 1.2.1.60)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.60

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (490 amino acids)

>GFF1827 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (EC 1.2.1.60) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MTTIQHLINGKAVASAASFETLNPATQDVLAEVSAGGAAEINAAVQAAKDAFPKWAGLPA
TERARLMRKLGELIAAHVPEIAQTETNDSGQVIAQTGKQLVPRAADNFHYFAEMCTRVDG
HTYPTPTHLNYTLFHPVGVCALISPWNVPFMTATWKVAPCLAFGNTAVLKMSELSPLTAA
RLGELALEAGIPAGVLNLVHGYGKDAGEPLCAHPDVRAISFTGSTATGNRIVQSAGLKKF
SMELGGKSPFVIFDDADLGRALDAAVFMIFSNNGERCTAGSRILVQQSIYADFVAKFVER
AKRITVGDPLDEKTIVGPMISQAHLAKVRSYIELGPKEGATMLCGGLDRPSYAPELSSRV
AKGNYVWPTVFADVDNRMRIAQEEIFGPVACLIPFRDEAHAIELANDIQYGLSSYVWTEN
LGRAHRVAAAVEAGMCFVNSQNVRDLRQPFGGTKASGTGREGGTWSYEVFLEPKNVAVSL
GSHHIPHWGV