Protein Info for GFF1814 in Variovorax sp. SCN45

Annotation: 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU) decarboxylase (EC 4.1.1.97) / Allantoate amidohydrolase (EC 3.5.3.9)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 592 signal peptide" amino acids 1 to 17 (17 residues), see Phobius details PF09349: OHCU_decarbox" amino acids 9 to 170 (162 residues), 156.6 bits, see alignment E=1.5e-49 TIGR03164: OHCU decarboxylase" amino acids 16 to 171 (156 residues), 172.5 bits, see alignment E=6.8e-55 TIGR01879: amidase, hydantoinase/carbamoylase family" amino acids 193 to 577 (385 residues), 324.7 bits, see alignment E=8.6e-101 PF04389: Peptidase_M28" amino acids 239 to 324 (86 residues), 21.5 bits, see alignment E=3.4e-08 PF01546: Peptidase_M20" amino acids 256 to 584 (329 residues), 68.7 bits, see alignment E=1.4e-22 PF07687: M20_dimer" amino acids 391 to 488 (98 residues), 22.6 bits, see alignment E=1.7e-08

Best Hits

KEGG orthology group: K06016, N-carbamoyl-L-amino-acid hydrolase [EC: 3.5.1.87] (inferred from 93% identity to vpe:Varpa_4541)

Predicted SEED Role

"N-carbamoyl-L-amino acid hydrolase (EC 3.5.1.87)" in subsystem Hydantoin metabolism (EC 3.5.1.87)

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.87

Use Curated BLAST to search for 3.5.1.87

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (592 amino acids)

>GFF1814 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU) decarboxylase (EC 4.1.1.97) / Allantoate amidohydrolase (EC 3.5.3.9) (Variovorax sp. SCN45)
MSLTIAQLNAAVPATAVALLDGIYEHSPWIAQRALAARPFRSLAHLKHALVRALAESSAD
EQIGLIRAHPELAGKAMVSKTLTAESTNEQSKAGLTDCTPEEFAKIQQLNADYNAKFGFP
FILAVRGPRGAGLAKREIIETFARRLHNHPAFELGEALRNIHRIAEIRLDDKFAADISLG
NDVWDWQEQLSVHTDPGYAEKGQLTVTYLTDAHRACAAQISALMSDVGFDSVHIDAVGNV
VGRYEATQPGARTLLTGSHYDTVRNGGKYDGRLGIFVPIACVRELKRQNKRLPFAFEVVG
FAEEEGQRYKATFLGSGALIGHFDQRWLDQKDADGVTMREAMQHAGLKQEDIPKIQRDPA
RYLGFVEVHIEQGPVLTELDLPLGIVTSINGSVRYVGEVIGMASHAGTTPMDRRRDAAAA
VAELILYTEQRAAKDGDSVGTVGMLEVPSGSINVVPGRCKFSLDLRAPNNAQRDALATDV
VGALKDICERRGVRFELEETMRAAAAPSAPAWQQRWEKAVDALGIPLFRMPSGAGHDAMK
LHEVMPQAMLFVRGINSGISHNPLESSTNDDIQLAVQAFQGLLDNLAAEQAH