Protein Info for GFF1812 in Xanthobacter sp. DMC5

Annotation: putative periplasmic iron-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 307 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details PF01297: ZnuA" amino acids 36 to 299 (264 residues), 288.7 bits, see alignment E=2.3e-90

Best Hits

Swiss-Prot: 66% identical to YFEA_YERPE: Periplasmic chelated iron-binding protein YfeA (yfeA) from Yersinia pestis

KEGG orthology group: K11604, manganese/iron transport system substrate-binding protein (inferred from 87% identity to xau:Xaut_1207)

Predicted SEED Role

"Manganese ABC transporter, periplasmic-binding protein SitA" in subsystem Transport of Manganese

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (307 amino acids)

>GFF1812 putative periplasmic iron-binding protein (Xanthobacter sp. DMC5)
VPDAFVLSRRHFPALLLAAAAVTFAAPARAAAPFKVVTTFTVIADMAKNVAGDAAQVESI
TKPGAEIHNYQPTPGDLIKAQGANLILWNGLNLELWFEKFFSRLKNVPSVVVSTGVEPMG
ITDGPYSGKPNPHAWMSPSAALIYVDNIRDALVKYDPANAATYTANAAAYKAKITAAIDP
IRAELEKVPAEQRWLVTSEGAFSYLARDFGLKALYLWPINADQQGTPQQVRRVVDLIRKD
HIPVIFSESTISAEPAKQVARETGARYGGVLYVDSLSDPSGPVPTYLDLLKVTSGTIAAG
LTGKVAP