Protein Info for GFF1805 in Xanthobacter sp. DMC5

Annotation: N-carbamoyl-L-amino acid hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01879: amidase, hydantoinase/carbamoylase family
  • amino acids 33 to 417 (385 residues), 360.5 bits, E=5.8e-112
• PF04389: Peptidase_M28
  • amino acids 73 to 153 (81 residues), 27.4 bits, E=4.1e-10
• PF01546: Peptidase_M20
  • amino acids 89 to 416 (328 residues), 79.3 bits, E=6e-26
• PF07687: M20_dimer
  • amino acids 221 to 323 (103 residues), 37.9 bits, E=2.3e-13

Best Hits

Swiss-Prot: 40% identical to AMAB2_GEOSE: N-carbamoyl-L-amino acid hydrolase (amaB) from Geobacillus stearothermophilus

KEGG orthology group: K02083, allantoate deiminase [EC: 3.5.3.9] (inferred from 85% identity to xau:Xaut_3289)

Predicted SEED Role

"N-carbamoyl-L-amino acid hydrolase (EC 3.5.1.87)" in subsystem Hydantoin metabolism (EC 3.5.1.87)

MetaCyc Pathways

• allantoin degradation IV (anaerobic) (6/9 steps found)
• allantoin degradation to glyoxylate II (3/5 steps found)
• allantoin degradation to ureidoglycolate II (ammonia producing) (2/4 steps found)
• allantoin degradation to glyoxylate III (2/5 steps found)
• superpathway of allantoin degradation in plants (4/8 steps found)

KEGG Metabolic Maps

• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.87

Use Curated BLAST to search for 3.5.1.87 or 3.5.3.9

Protein Sequence (422 amino acids)

>GFF1805 N-carbamoyl-L-amino acid hydrolase (Xanthobacter sp. DMC5)
VSRSAPGSAPLPSPLDGEVIVSRLKELAACTDVPGEMTRLTLTPAHKRAAAQVSFWMREA
GLAQVHMDATGSIVGRYAADRANAKTLVLGSHIDTVRNAGIYDGNLGVLTAIAAVEALKQ
QGIRLPFAIEVAAFADEEGVRFLSTLTSSKAMAGIFDPKSLEDVDAQGISRMEALRSFGA
PVDRVADCRRDPAETLGYVEVHIEQGPVLEAAGAPLGIVTGIAGASRGHVRIRGEAGHSG
TLPMAMRHDALTAAAEMILAVEARGRADGKELVATVGTLLIAGSAVNVVPGEVQFSLDVR
ALSDELRIRAVADIRAAVADIAERRGVKASVDIGHEVPAAPCHGPLKERMAKVVEGLQLP
VVRLPSGAGHDAMVFRGVMPMAMLFVRSENGSHNPREYAAPADIGLAAEALYAFLLKTAE
EM