Protein Info for PS417_00090 in Pseudomonas simiae WCS417

Annotation: methionyl-tRNA formyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 317 TIGR00460: methionyl-tRNA formyltransferase" amino acids 5 to 305 (301 residues), 320 bits, see alignment E=7.5e-100 PF00551: Formyl_trans_N" amino acids 6 to 184 (179 residues), 162.6 bits, see alignment E=8.8e-52 PF02911: Formyl_trans_C" amino acids 207 to 302 (96 residues), 96.4 bits, see alignment E=1e-31

Best Hits

Swiss-Prot: 96% identical to FMT_PSEFS: Methionyl-tRNA formyltransferase (fmt) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K00604, methionyl-tRNA formyltransferase [EC: 2.1.2.9] (inferred from 96% identity to pfs:PFLU0017)

MetaCyc: 58% identical to 10-formyltetrahydrofolate:L-methionyl-tRNAfMet N-formyltransferase (Escherichia coli K-12 substr. MG1655)
Methionyl-tRNA formyltransferase. [EC: 2.1.2.9]; 2.1.2.9 [EC: 2.1.2.9]

Predicted SEED Role

"Methionyl-tRNA formyltransferase (EC 2.1.2.9)" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or Folate Biosynthesis (EC 2.1.2.9)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.2.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7TV22 at UniProt or InterPro

Protein Sequence (317 amino acids)

>PS417_00090 methionyl-tRNA formyltransferase (Pseudomonas simiae WCS417)
MTEPLRIVFAGTPEFAAEHLKALLASPYDIVAVYTQPDRPAGRGQKLMPSPVKQLALEHD
IPVLQPPTLRNAEAQAELAALKPDLLVVVAYGLILPQVVLDIPRLGCINSHASLLPRWRG
AAPIQRAVEAGDSESGVTVMRMEAGLDTGPMLLKVTTPITAQDTGGSLHDRLAELGPPAV
IQAIAGLAAGTLQGDVQDDSLATYAHKLNKDEARIDWRRPAVELERLVRAFNPWPICHST
LNGEALKVLAATLAEGKGAPGEIIGASKDGLVVACGEQALCLTRLQLPGGKALNFSDLFN
SRREKFALGTVLGVVAQ