Protein Info for GFF1792 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: DNA-directed RNA polymerase beta' subunit (EC 2.7.7.6)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1406 PF04997: RNA_pol_Rpb1_1" amino acids 16 to 340 (325 residues), 269 bits, see alignment E=1.5e-83 TIGR02386: DNA-directed RNA polymerase, beta' subunit" amino acids 19 to 1365 (1347 residues), 1780.9 bits, see alignment E=0 PF00623: RNA_pol_Rpb1_2" amino acids 342 to 393 (52 residues), 33.6 bits, see alignment (E = 1.2e-11) amino acids 391 to 483 (93 residues), 95.8 bits, see alignment 9.1e-31 PF04983: RNA_pol_Rpb1_3" amino acids 487 to 643 (157 residues), 93.1 bits, see alignment E=4.6e-30 PF05000: RNA_pol_Rpb1_4" amino acids 673 to 763 (91 residues), 77.7 bits, see alignment 1.5e-25 PF04998: RNA_pol_Rpb1_5" amino acids 765 to 1314 (550 residues), 256.9 bits, see alignment E=5.8e-80

Best Hits

Swiss-Prot: 90% identical to RPOC_ACIAC: DNA-directed RNA polymerase subunit beta' (rpoC) from Acidovorax citrulli (strain AAC00-1)

KEGG orthology group: K03046, DNA-directed RNA polymerase subunit beta' [EC: 2.7.7.6] (inferred from 90% identity to aav:Aave_4530)

Predicted SEED Role

"DNA-directed RNA polymerase beta' subunit (EC 2.7.7.6)" in subsystem RNA polymerase bacterial (EC 2.7.7.6)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.6

Use Curated BLAST to search for 2.7.7.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1406 amino acids)

>GFF1792 DNA-directed RNA polymerase beta' subunit (EC 2.7.7.6) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MKSLLDLFKQFTPDEHFDAIRIGLASPEKIRSWSFGEVKKPETINYRTFKPERDGLFCAK
IFGPIKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERMGHIDLAAPCAHIWFLKSL
PSRLGLILDMTLRDIERVLYFEAYVIVDPGMTPLKKFAIMSEDDYDAKRKEFGDEFIAKM
GAEGIKELLQAIDLDIEIEKLRNDLTGSELKIKKNAKRLKVLEAFKKSGIKPEWMVLDVL
PVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRNSRLRRLLELKAPEIIARNEKRMLQEA
VDSLLDNGRRGKAMTGANKRALKSLADMIKGKSGRFRQNLLGKRVDYSGRSVIVVGPTLK
LHQCGLPKLMALELFKPFIFSRLEAMGIATTIKAAKKEVEAGTPVVWDILEEVIKEHPVM
LNRAPTLHRLGIQAFEPILIEGKAIQLHPLVCAAFNADFDGDQMAVHVPLSVEAQLEART
LMLASNNILFPANGEPSIVPSQDVVLGLYYATRERINAKGEGMLFADLAELQRALDNGVV
EITAKIGVRLTQHALDKASGEFVASTSLVDTTVGRALLSEILPKGLPFDVLNKALKKKEI
SRLINTSFRKCGLKETVVFADKLLQNGFRLATRAGISIAVDDMLVPKEKPAIIDRAENEV
KEIAQQYASGLVTAGERYNKVVDIWGKAGDEISKVMMAQLAKQKVTDSSGKEVDQESFNS
IYMMADSGARGSAAQIRQLAGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARK
GLADTALKTANSGYLTRRLVDVTQDLVVNSQDCGTSNGTLMRAIVEGGEVIESLRDRVLG
RTTAEEVIHPETREVLVPAGELLNEDLLDVLEAAAVDEVKVRTALTCETRFGICAKCYGR
DLGRGGLVNIGEAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAVASSVEAKSSGVIGFN
ATMRYVTNTKGELVVIARSGEIVIHDEHGRERERHKVPYGAILSVKADQTIKAGAILANW
DPLTRPIITEFAGQVKFENVEEGLTVAKQVDDVTGLSTLVVIDPKRRGAAKVVRPQVKLI
DGTGNEVKIPGTDHSVTIGFQIGSLIQVRDGQDVGPGEVLARIPVEGQKTRDITGGLPRV
AELFEARSPKDKGMLAEMTGTVSFGKETKGKVRLQITDPDGKVWDELIPKEKNILVHEGQ
VVNKGESIVDGPADPQDILRLLGIEELSRYIVDEVQDVYRLQGVKINDKHIEVIVRQMLR
RVVVEAVGDSSYIAGEQVERSEILNTNDALRADGKIPATYSNVLLGITKASLSTDSFISA
ASFQETTRVLTEAAIMGKRDELRGLKENVIVGRLIPAGTGMAYHEARKVKEKMDDEERRA
IAEADAISLAADQADQADASSTHEAE