Protein Info for GFF179 in Variovorax sp. SCN45

Annotation: D-mannonate oxidoreductase (EC 1.1.1.57)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 PF01232: Mannitol_dh" amino acids 29 to 195 (167 residues), 117.4 bits, see alignment E=6.7e-38 PF08125: Mannitol_dh_C" amino acids 224 to 444 (221 residues), 266.5 bits, see alignment E=2.3e-83

Best Hits

KEGG orthology group: K00040, fructuronate reductase [EC: 1.1.1.57] (inferred from 69% identity to vap:Vapar_2884)

Predicted SEED Role

"D-mannonate oxidoreductase (EC 1.1.1.57)" in subsystem D-Galacturonate and D-Glucuronate Utilization (EC 1.1.1.57)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.57

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (500 amino acids)

>GFF179 D-mannonate oxidoreductase (EC 1.1.1.57) (Variovorax sp. SCN45)
MPRLHPDALPHVPAHVAVPRYRRESLRAGIVHLGVGAFHRAHLAVVNEAALHAGDDLRWG
TVGVSLRAPDTRDALKPQAGLYTLALRDAAEDGSPRELLQVVGNLLDVLVAPEDPRAVLE
RIAHPDTRIVSLTITEKGYHHDPATGALKLDDVGIAHDLAHPEAPRTMPGFVVHAMALRR
ARGLAPVTLLSCDNLPANGDTLRGIVLAFAQAVDVALRDWIDAHCTFPNSMVDRIVPRTT
NEDRARISERLGVEDAWPVIGEPFLEWVVEDRFASGRPDWTAGGARFVAHAEPFERLKLR
MVNGSHSALAYLGAMAGFRTVDRAMAEPALRAFIDALMREEIAPTLPAMEGLDLDGYRAR
LLERFSNPALRHQTKQIAMDGSQKLPQRLLGTVRDRLAAGLPIDRLALAVAGWVHYLRGV
DETGAAHEIQDPLASELARRLARANAASSMQDRVAFFTGFAPVFGELGGEPRFVAAVAQQ
LGVLETQGVRGALAASTASA