Protein Info for GFF1788 in Methylophilus sp. DMC18

Annotation: Chaperone protein ClpB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 878 TIGR03346: ATP-dependent chaperone protein ClpB" amino acids 24 to 875 (852 residues), 1446.7 bits, see alignment E=0 PF02861: Clp_N" amino acids 35 to 86 (52 residues), 37.7 bits, see alignment 1.5e-12 amino acids 113 to 163 (51 residues), 44.8 bits, see alignment 8.9e-15 PF00004: AAA" amino acids 221 to 337 (117 residues), 51.3 bits, see alignment E=1.3e-16 amino acids 623 to 742 (120 residues), 40.7 bits, see alignment E=2.5e-13 PF17871: AAA_lid_9" amino acids 360 to 458 (99 residues), 118.7 bits, see alignment E=8.1e-38 PF07724: AAA_2" amino acids 617 to 780 (164 residues), 242.7 bits, see alignment E=1.7e-75 PF00158: Sigma54_activat" amino acids 621 to 747 (127 residues), 25.9 bits, see alignment E=5.9e-09 PF07728: AAA_5" amino acids 622 to 743 (122 residues), 51 bits, see alignment E=1.3e-16 PF10431: ClpB_D2-small" amino acids 786 to 866 (81 residues), 93.7 bits, see alignment E=4.6e-30

Best Hits

Swiss-Prot: 80% identical to CLPB_NITEU: Chaperone protein ClpB (clpB) from Nitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298)

KEGG orthology group: K03695, ATP-dependent Clp protease ATP-binding subunit ClpB (inferred from 80% identity to azo:azo1566)

MetaCyc: 67% identical to protein disaggregase ClpB (Escherichia coli K-12 substr. MG1655)
RXN185E-10

Predicted SEED Role

"ClpB protein" in subsystem Protein chaperones or Proteolysis in bacteria, ATP-dependent

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (878 amino acids)

>GFF1788 Chaperone protein ClpB (Methylophilus sp. DMC18)
MGIQPQACGDTLIKKGFAMRFDKLTTKFQQALADAQSMAVGADHPTIEAPHLLLAMLNQD
DSGVAALLSRAGAQVSALKTGLQQAIDNLPKVSENSGDVSVSRDLNNLLNVTDKLAQKSG
DSYIASEMFLLALAEDKGPTGQLLKQHGLSKTALEQAIKAVRGNDSVNSQEAEGQREALK
KYTLDLTERARAGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQR
IVNGEVPDSLKNKKVLSLDMAALLAGAKYRGEFEERLKAVLKELAQDEGQTIVFIDEIHT
MVGAGKSEGAMDAGNMLKPALARGELHCVGATTLDEYRKYIEKDAALERRFQKVLVDEPS
VEATIAILRGLQEKYELHHGVEITDPAIVAAAELSNRYITDRFLPDKAIDLIDEAASRIK
MEIDSKPEVMDKLDRRLIQLKIEREAVRREKDEASQKRFGLIEEEISKLEKEYADLEEIW
KAEKAAVQGSAGVKEAIEKIKLEMEAAKRKGDWQKVSELQYGKLPQLEAQLKHASSAEAS
GTVKHKMLRTEVGADEIAEVVSRATGIPVSKMMTGEREKLLNMENSLHARVVGQDEAVRL
VSDAIRRSRSGLSDPNRPYGSFLFLGPTGVGKTELCKALANFLFDSEDHLVRIDMSEFME
KHSVSRLIGAPPGYVGYEEGGTLTEAVRRKPYSVVLLDEVEKAHPDVFNVLLQVLDDGRL
TDGQGRTVDFKNTVIIMTSNLGSQMIQSMSDQDYQVVKLAVMGEVKTHFRPEFINRIDEV
VVFHSLGEAHVKSIANIQLQLLAKRLAAMEMQLDVTDAALAEIANAGFDPVYGARPLKRA
IQSEIENPLAREILAGQFAAKDTIQVDFQGGKMQFRKA