Protein Info for GFF1785 in Methylophilus sp. DMC18

Annotation: Cyclic di-GMP phosphodiesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 425 PF11871: DUF3391" amino acids 11 to 128 (118 residues), 77.6 bits, see alignment E=2.1e-25 TIGR00277: HDIG domain" amino acids 173 to 264 (92 residues), 38.1 bits, see alignment E=5.4e-14 PF01966: HD" amino acids 175 to 295 (121 residues), 54.1 bits, see alignment E=2.9e-18 PF13487: HD_5" amino acids 184 to 327 (144 residues), 95.8 bits, see alignment E=3.9e-31

Best Hits

KEGG orthology group: None (inferred from 39% identity to pfv:Psefu_4198)

Predicted SEED Role

"metal dependent phosphohydrolase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (425 amino acids)

>GFF1785 Cyclic di-GMP phosphodiesterase (Methylophilus sp. DMC18)
MHNQASDTYYISPDQLQVGLYIHLDLGWMDHPFSVSNFKITEDAQIDTIKSLGLKQLRYD
PRRSTSHPLKKNANVVEFLVKKALFPEPEPAKETTPLNAAQQKRQVLKQVINESEQKFMR
TSAEVKNIQKLSTEHPDQAYGLAQTLVQDLVSSTLTEGDIAIHAMSGHRVGDQHYQHELN
TLVASLILAKNLNISDEDAVILGMAAILHDIGKRQISDKILLKKDPLNLREVAIYQTHVA
LGLNMLKEVAVPKKIMTLIAQHHEFADGSGYPQGLRAEQIDPLSHILIIANVYDNLCNPS
NPALAKTPYEALSMMFAQQRHQFDQTILRRFIKCLGIYPPGSVVRLSDQQLATVVSTNPQ
QPLRPFVQILNDDPEEEGETFDLREALDINIIQCLKPEQLPDILQKRMRLKQRTSYFLDK
SLALM