Protein Info for GFF178 in Variovorax sp. SCN45

Annotation: Mannonate dehydratase (EC 4.2.1.8)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 403 PF02746: MR_MLE_N" amino acids 16 to 110 (95 residues), 111.2 bits, see alignment E=3.6e-36 PF13378: MR_MLE_C" amino acids 142 to 377 (236 residues), 152.5 bits, see alignment E=1.4e-48

Best Hits

Swiss-Prot: 81% identical to MAND_SPHWW: D-mannonate dehydratase (Swit_1693) from Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273)

KEGG orthology group: K08323, starvation sensing protein RspA (inferred from 90% identity to vap:Vapar_2883)

Predicted SEED Role

"Starvation sensing protein RspA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (403 amino acids)

>GFF178 Mannonate dehydratase (EC 4.2.1.8) (Variovorax sp. SCN45)
MKITNARVIVCSPGRNFVTLKIETDEGLTGIGDATLNGRELAVAAYLTEHVIPCLIGRDA
HQIEDIWQYLYKGAYWRRGPVTMTAIAAVDTALWDIKAKAANMPLYQLLGGKSRTGVMVY
GHANGRDIEHTVDEVLRYKEEGYLAIRAQSGVPGLEKVYGVGRGTMFYEPADADLPSEHD
WSTAKYLEHTPKLFDAVRSAVGPDIHLLHDVHHRLTPIEAGRLGKSLEPFNLFWMEDATP
AENQEAFRLIRQHTTTPLAVGEIFNSIWDCKDLIENQLIDFIRTTVVHAGGITQLRRIAD
LASLYQVRTGCHGATDLSPACMGAALHFDMWVPNFGIQEYMRHTEETDAVFPHSYSFSNG
MLHPGEAPGHGVDIDEALAAKYPYKRAYLPVNRLQHDGTLWNW