Protein Info for GFF1769 in Methylophilus sp. DMC18

Annotation: N5-carboxyaminoimidazole ribonucleotide synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 382 PF22660: RS_preATP-grasp-like" amino acids 12 to 106 (95 residues), 106 bits, see alignment E=2.2e-34 TIGR01161: phosphoribosylaminoimidazole carboxylase, ATPase subunit" amino acids 12 to 367 (356 residues), 391.2 bits, see alignment E=2.6e-121 PF02222: ATP-grasp" amino acids 114 to 287 (174 residues), 180.3 bits, see alignment E=5.8e-57 PF02786: CPSase_L_D2" amino acids 134 to 299 (166 residues), 21 bits, see alignment E=4.2e-08 PF17769: PurK_C" amino acids 308 to 368 (61 residues), 66.5 bits, see alignment E=2.8e-22

Best Hits

Swiss-Prot: 43% identical to PURK_BACSU: N5-carboxyaminoimidazole ribonucleotide synthase (purK) from Bacillus subtilis (strain 168)

KEGG orthology group: K01589, 5-(carboxyamino)imidazole ribonucleotide synthase [EC: 6.3.4.18] (inferred from 72% identity to mmb:Mmol_0526)

Predicted SEED Role

"Phosphoribosylaminoimidazole carboxylase ATPase subunit (EC 4.1.1.21)" in subsystem De Novo Purine Biosynthesis (EC 4.1.1.21)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.1.1.21

Use Curated BLAST to search for 4.1.1.21 or 6.3.4.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (382 amino acids)

>GFF1769 N5-carboxyaminoimidazole ribonucleotide synthase (Methylophilus sp. DMC18)
MTSSVIQPPAMLGMLGGGQLGRFFVIAAHEMGYKVTVLDPDQNSPAGMIADVHLCAAYDD
AAALKTMAETCQAISTEFENVPAETLATLAKSTVVHPSADAVAIAQHRVKEKTFFKEAGL
PVGPFVPVMAESDLPADGEMYPAVLKVARFGYDGKGQARVANREQAVAAFQGFKQEVCVL
EKMLPLDLEVSVVLARDAHGHIAAFPTAENSHLNGILDVSIAPARCSEVIKANAQELAKK
LATKLNYVGVLGVEFFVVGNQLLVNEIAPRPHNSGHYTIDACVTNQFEQQVRVITGQALG
SPRLHSDAVMVNILGDSWLYSGKQVEPAWDKAFAHGNLKMHLYGKHEPRKGRKMGHFTVI
GDDAQAVLNAAKVARSELHIGE