Protein Info for GFF176 in Variovorax sp. SCN45

Annotation: ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 219 transmembrane" amino acids 20 to 43 (24 residues), see Phobius details amino acids 58 to 78 (21 residues), see Phobius details amino acids 80 to 82 (3 residues), see Phobius details amino acids 84 to 84 (1 residues), see Phobius details amino acids 86 to 105 (20 residues), see Phobius details amino acids 164 to 164 (1 residues), see Phobius details amino acids 184 to 203 (20 residues), see Phobius details TIGR01726: amino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family" amino acids 14 to 110 (97 residues), 91.9 bits, see alignment E=1.5e-30 PF00528: BPD_transp_1" amino acids 33 to 214 (182 residues), 87.5 bits, see alignment E=4.8e-29

Best Hits

Swiss-Prot: 39% identical to YECS_ECOL6: L-cystine transport system permease protein YecS (yecS) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K10037, glutamine transport system permease protein (inferred from 76% identity to vap:Vapar_2881)

MetaCyc: 39% identical to cystine ABC transporter membrane subunit (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-290 [EC: 7.4.2.12]; 7.4.2.12 [EC: 7.4.2.12]; 7.4.2.- [EC: 7.4.2.12]; 7.4.2.- [EC: 7.4.2.12]; 7.4.2.- [EC: 7.4.2.12]; 7.4.2.- [EC: 7.4.2.12]

Predicted SEED Role

"cystine ABC tranporter, permease protein, putative"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (219 amino acids)

>GFF176 ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines) (Variovorax sp. SCN45)
MELDWNIVWTHRYDLLHGAGLTVLLTVLTMLVAVPLGIVLALMRLSSSKALSGASQVFVE
FFRNLPLILVVYWAFYVMPVLMHIEFSAFTTGLVALCLNVSAYNAETFRAGINSIRKGQM
EAAIAMGMSRWQAMKKVVIPQAWRRVLPVLASTWVSLFKDTSLVSVIAVGELAHVALQIR
SSSFRVLEMLTAMAVIYWLMGYPQAKLVDWIQKKFEVKE