Protein Info for GFF1745 in Sphingobium sp. HT1-2

Annotation: Cytochrome d ubiquinol oxidase subunit I (EC 1.10.3.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 526 transmembrane" amino acids 19 to 44 (26 residues), see Phobius details amino acids 56 to 75 (20 residues), see Phobius details amino acids 94 to 118 (25 residues), see Phobius details amino acids 129 to 149 (21 residues), see Phobius details amino acids 190 to 210 (21 residues), see Phobius details amino acids 222 to 240 (19 residues), see Phobius details amino acids 388 to 411 (24 residues), see Phobius details amino acids 423 to 445 (23 residues), see Phobius details amino acids 472 to 495 (24 residues), see Phobius details PF01654: Cyt_bd_oxida_I" amino acids 9 to 502 (494 residues), 572.7 bits, see alignment E=1.9e-176

Best Hits

Swiss-Prot: 58% identical to CYDA_AZOVI: Cytochrome bd ubiquinol oxidase subunit 1 (cydA) from Azotobacter vinelandii

KEGG orthology group: K00425, cytochrome bd-I oxidase subunit I [EC: 1.10.3.-] (inferred from 78% identity to swi:Swit_3736)

MetaCyc: 56% identical to cytochrome bd-I subunit 1 (Escherichia coli K-12 substr. MG1655)
RXN0-5266 [EC: 7.1.1.7]; 1.11.1.- [EC: 7.1.1.7]; 1.11.1.- [EC: 7.1.1.7]

Predicted SEED Role

"Cytochrome d ubiquinol oxidase subunit I (EC 1.10.3.-)" in subsystem Terminal cytochrome d ubiquinol oxidases or Terminal cytochrome oxidases (EC 1.10.3.-)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.10.3.-

Use Curated BLAST to search for 1.10.3.- or 7.1.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (526 amino acids)

>GFF1745 Cytochrome d ubiquinol oxidase subunit I (EC 1.10.3.-) (Sphingobium sp. HT1-2)
MISMAVVELSRLQFALTALYHFLFVPLTLGLSFMLVIMESVYVMTDRPIWRDITRFWGKL
FGINFVLGVATGLTMEFEFGTNWAYYSHYVGDIFGAPLAIEGLMAFFLEATFVGLMFFGW
DRLSKRGHLLTTFMVALGSNLSALWILVANGWMQHPTGATFNPQTMRMEVTDFMAVIFNP
VAQAKFVHTVSAGYVCASVFVLGVSAFYLLKGKHIAIAKRSFTVAAAFGLAASLSVVVLG
DESGYALTENQKMKLAAIEGMWQTEPAPAGLTLFGLPSNSGRETTHEIKVPYVLGLISTR
SLTGEVTGINQLVDTAKVRIAAGIIAYDAVETLKVDRNDLVAREAFERNKADLGYALLLK
RFIADPRQADAATIEKAAWSTVPNVPAIFWMFRIMAGLGFAFIALFAIAFAFSSMRRFDA
RWFLKLALFMMPMPWIAIEVGWAVAEIGRQPWAVDGVLPTFLAASSLTVPQLWTTIIGFT
LLYGILAVIEVKLMLATIAKGPERYVPWRPDSPAEPAPDFRAVPAE