Protein Info for GFF1744 in Methylophilus sp. DMC18

Annotation: Queuine tRNA-ribosyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00449: tRNA-guanine family transglycosylase
  • amino acids 3 to 191 (189 residues), 251.4 bits, E=1.3e-78
  • amino acids 204 to 393 (190 residues), 287.1 bits, E=1.8e-89
• PF01702: TGT
  • amino acids 10 to 393 (384 residues), 557.5 bits, E=6.3e-172

Predicted SEED Role

"tRNA-guanine transglycosylase (EC 2.4.2.29)" in subsystem Queuosine-Archaeosine Biosynthesis (EC 2.4.2.29)

MetaCyc Pathways

• queuosine biosynthesis I (de novo) (4/4 steps found)
• queuosine biosynthesis III (queuosine salvage) (3/5 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.2.29

Protein Sequence (396 amino acids)

>GFF1744 Queuine tRNA-ribosyltransferase (Methylophilus sp. DMC18)
MQFSLHNTDGLARRGTVSLPHGEVQTPAFMPVGTYGTVKAMSPQELNEIQAHIVLGNTFH
LWLRPGLEVIAAHDGLHKFMGWDKPILTDSGGFQVWSLGDLRKITEEGVKFQSPINGDRC
FLTPEESMRIQRVLNSDIVMIFDECTPYPASHDQARKSMELSLRWAQRSRDAFNVYQTHT
IAHTLTLQGGQEQVAQLRAEHLPTNGNALFGIIQGGMYEDLRDISRQGLEAIGFDGYAIG
GLSVGEPKEDMLRILAHTAPQMPKDKPRYLMGVGTPEDLVDAVSYGIDMFDCVMPTRNAR
NGWLFTQYGDVKIKNASYKTDTRPLDDECECYTCQHFSRAYLHHLHRIGEILGARLNTIH
NLHYYQVLMQGMRAAIEENRFEAFKAEFARKRAEKR