Protein Info for GFF1729 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01173: UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase
  • amino acids 6 to 455 (450 residues), 678.7 bits, E=1.9e-208
• PF01128: IspD
  • amino acids 6 to 50 (45 residues), 28.7 bits, 2.7e-10
• PF12804: NTP_transf_3
  • amino acids 8 to 121 (114 residues), 72.2 bits, E=1.4e-23
• PF00483: NTP_transferase
  • amino acids 8 to 213 (206 residues), 52.3 bits, E=1.5e-17
• PF14602: Hexapep_2
  • amino acids 395 to 429 (35 residues), 30.5 bits, 6.3e-11
• PF00132: Hexapep
  • amino acids 395 to 429 (35 residues), 35 bits, 2e-12

Best Hits

Swiss-Prot: 100% identical to GLMU_SALHS: Bifunctional protein GlmU (glmU) from Salmonella heidelberg (strain SL476)

KEGG orthology group: K04042, bifunctional UDP-N-acetylglucosamine pyrophosphorylase / Glucosamine-1-phosphate N-acetyltransferase [EC: 2.3.1.157 2.7.7.23] (inferred from 99% identity to seg:SG3571)

MetaCyc: 91% identical to fused N-acetylglucosamine-1-phosphate uridyltransferase and glucosamine-1-phosphate acetyltransferase (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylglucosamine diphosphorylase. [EC: 2.7.7.23]; Glucosamine-1-phosphate N-acetyltransferase. [EC: 2.7.7.23, 2.3.1.157]

Predicted SEED Role

"N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)" in subsystem Peptidoglycan Biosynthesis or Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.3.1.157, EC 2.7.7.23)

MetaCyc Pathways

• peptidoglycan recycling I (14/14 steps found)
• O-antigen building blocks biosynthesis (E. coli) (11/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (5/7 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• chitin biosynthesis (6/9 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (14/33 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (7/24 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.157 or 2.7.7.23

Protein Sequence (456 amino acids)

>GFF1729 N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MLNSAMSVVILAAGKGTRMYSDIPKVLHTLAGKPMVQHVIDAATKLGAAQVHLVYGHGGE
LLKQTLKDDKLNWVLQAEQLGTGHAMQQAAPFFSDDEDILMLYGDVPLISVETLQRLRDA
KPQGGIGLLTVKLDDPSGYGRITRENGKVTGIVEHKDATDEQRQIQEINTGILIANGADM
KRWLSKLTNNNAQGEYYITDIIALAYQEGREIAAVHPARISETDGVNNRLQLSRLERIYQ
AEQAEKLLLSGVMLRDPARFDLRGTLHCGMDVEIDANVIIEGYVTLGHRVKIGAGCIIKN
SVIGDDCEISPYSVVEDAHLEAACTIGPFARLRPGAELLAGAHVGNFVEMKKARLGKGSK
AGHLTYLGDAEIGDNVNIGAGTITCNYDGANKFKTVIGDDVFVGSDTQLVAPVTVGKGAT
IAAGTTVTRNVADNELVLSRVPQVHKQGWQRPVKKK