Protein Info for GFF1715 in Hydrogenophaga sp. GW460-11-11-14-LB1
Annotation: Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 66% identical to MCCB_MOUSE: Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial (Mccc2) from Mus musculus
KEGG orthology group: K01969, 3-methylcrotonyl-CoA carboxylase beta subunit [EC: 6.4.1.4] (inferred from 85% identity to hmg:100207816)MetaCyc: 74% identical to methylcrotonyl-CoA carboxylase beta-subunit (Pseudomonas aeruginosa PAO1)
Methylcrotonoyl-CoA carboxylase. [EC: 6.4.1.4]
Predicted SEED Role
"Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism or Serine-glyoxylate cycle (EC 6.4.1.4)
MetaCyc Pathways
- L-leucine degradation I (5/6 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.4.1.4
Use Curated BLAST to search for 6.4.1.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (540 amino acids)
>GFF1715 Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4) (Hydrogenophaga sp. GW460-11-11-14-LB1) MPILETQLNARSADFQANAQAMQVVVDDLKAQIAKATLGGGEAARAKHTARGKLLPRDRV QMLLDPGTPFLELSPLAALNMYPDRDGTDSAPCAGVIAGIGRVSGVDCLIVCNDATVKGG TYYPMTVKKHLRAQEVAMQNNLTCIYLVDSGGANLPNQDDVFPDREHFGRIFYNQANMSA NGIAQIAVVMGSCTAGGAYVPAMSDETIIVKNQGTIFLGGPPLVKAAIGEIVSAEDLGGG DVHTRLSGVADHLAQNDAHALALARQAVATLNRRKVATQALREPRAPLNDPKEIYGVIPV DTRKPYDVREIIARVVDGSEFHEFKARYGATLVCGFAHIEGMPIGIVANNGVLFSESAQK GAHFIELCCQRKVPLLFLQNITGFMVGRKYESEGIARHGAKMVTAVATAQVPKFTIIIGG SYGAGNYGMCGRAYSPRFLWMWPNARISVMGGEQAASVLATVRRDGIEGKGGSWSAEEEA KFKQPLLDQFAHQSHPYYASARLWDDGVIDPADTRRVLGLALSATLNTPIGEPKFGVFRM