Protein Info for GFF1713 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Methylglutaconyl-CoA hydratase (EC 4.2.1.18)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00378: ECH_1
  • amino acids 14 to 259 (246 residues), 155.3 bits, E=1.9e-49
• PF16113: ECH_2
  • amino acids 16 to 235 (220 residues), 84.4 bits, E=1.1e-27

Best Hits

KEGG orthology group: K13766, methylglutaconyl-CoA hydratase [EC: 4.2.1.18] (inferred from 68% identity to lch:Lcho_2117)

MetaCyc: 41% identical to 3-methylglutaconyl-CoA hydratase (Pseudomonas putida)
Methylglutaconyl-CoA hydratase. [EC: 4.2.1.18]

Predicted SEED Role

"Methylglutaconyl-CoA hydratase (EC 4.2.1.18)" in subsystem Benzoate transport and degradation cluster or HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism (EC 4.2.1.18)

MetaCyc Pathways

• L-leucine degradation I (5/6 steps found)
• ferrichrome A biosynthesis (2/5 steps found)

KEGG Metabolic Maps

• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.18

Use Curated BLAST to search for 4.2.1.18

Protein Sequence (263 amino acids)

>GFF1713 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MSDVIDVSRPTPHVAKVWLNRPDVRNAFNDDVIAALTATFTELSKDKTLRVVVLGAHGKA
FCAGADLNWMKTMAGYSWDQNRADAQQLADMLWTLDQCPVPVVGRIQGDCYAGGMGLAAI
CDVLVAVEGATFCLSEARLGLLPATISPYVARALGVQASRRYMVTAERFSAVRAHALGMV
HELATPETLDAKVDEIVATLVANGPKAARECKRLVRDVGGQPITEALRSETARRIADVRA
SDEGREGLQSFLQKRPPGWLPTA