Protein Info for GFF1697 in Variovorax sp. SCN45

Annotation: Argininosuccinate lyase-like protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00838: argininosuccinate lyase
  • amino acids 14 to 468 (455 residues), 335.3 bits, E=3.4e-104
• PF00206: Lyase_1
  • amino acids 50 to 303 (254 residues), 141.9 bits, E=3.3e-45
• PF14698: ASL_C2
  • amino acids 369 to 445 (77 residues), 39.4 bits, E=6.6e-14

Best Hits

KEGG orthology group: K01755, argininosuccinate lyase [EC: 4.3.2.1] (inferred from 72% identity to pol:Bpro_4391)

Predicted SEED Role

"Argininosuccinate lyase (EC 4.3.2.1)" in subsystem Arginine Biosynthesis extended (EC 4.3.2.1)

MetaCyc Pathways

• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• superpathway of arginine and polyamine biosynthesis (15/17 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• urea cycle (4/5 steps found)
• nitric oxide biosynthesis II (mammals) (2/3 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• canavanine biosynthesis (2/4 steps found)
• L-arginine biosynthesis IV (archaea) (4/9 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 4.3.2.1

Use Curated BLAST to search for 4.3.2.1

Protein Sequence (507 amino acids)

>GFF1697 Argininosuccinate lyase-like protein (Variovorax sp. SCN45)
MTLHPEILLSDARLKSPAASRQLKYNELPKLQAERKKFDEFLAVDLAHTAMLVAQEIIAP
AAGRAILQQLLAIQEMGRDEFPVDVRKGSFLLQMESYLFAAIGEEVGGQMHTGRSRIDQG
ATVRRLYQRNRILDVMGRLNALQAAIAEQAEKHARTIMPGYTHMQQAQPWVFGHYLLSFS
SRLHDSFERLTQAYARVNRNPLGTVGLAGTSWPLDRALTTRLLGFDGLVENSKLGREAFY
AVDALSALSFVMADLNDLATDLHVWSSSEFGLVECDDSYCGTSSIFPQKKNPTALEYVRR
AAGASVTWVSTALATFRGEGTGDQAMREAPILDEALATAEAMLDLFTGVMETLIVHEDRM
AAALKESWCTSSNLADVIVRDCGLSFRQVHHIVARVVRNSLSESVAPYELTGAMVDRAAL
ETVGRPIGLSDSSVQTALNPREFVETRVTAGGVGPLEVRRLLAQAREQCAADMRWTDAAR
SALAEARCALDRAVGGILAPTSQPLGH