Protein Info for GFF1681 in Variovorax sp. SCN45

Annotation: Methylisocitrate lyase (EC 4.1.3.30)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13714: PEP_mutase
  • amino acids 7 to 249 (243 residues), 169.2 bits, E=1.2e-53
• PF00463: ICL
  • amino acids 76 to 178 (103 residues), 53.5 bits, E=1.6e-18

Best Hits

Swiss-Prot: 50% identical to DML_EUBBA: 2,3-dimethylmalate lyase (Dml) from Eubacterium barkeri

KEGG orthology group: K03417, methylisocitrate lyase [EC: 4.1.3.30] (inferred from 68% identity to adn:Alide_3498)

MetaCyc: 50% identical to 2,3-dimethylmalate lyase subunit (Eubacterium barkeri)
2,3-dimethylmalate lyase. [EC: 4.1.3.32]

Predicted SEED Role

"Methylisocitrate lyase (EC 4.1.3.30)" in subsystem Methylcitrate cycle or Propionate-CoA to Succinate Module (EC 4.1.3.30)

MetaCyc Pathways

• 2-methylcitrate cycle I (5/5 steps found)
• 2-methylcitrate cycle II (5/6 steps found)
• nicotinate degradation III (4/9 steps found)
• superpathway of nicotinate degradation (5/18 steps found)

KEGG Metabolic Maps

• Nicotinate and nicotinamide metabolism
• Propanoate metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.1.3.30

Use Curated BLAST to search for 4.1.3.30 or 4.1.3.32

Protein Sequence (290 amino acids)

>GFF1681 Methylisocitrate lyase (EC 4.1.3.30) (Variovorax sp. SCN45)
MSTPTSLRDLLGAPGMVIAPGAYDAIGARLIEQAGFAAAYMTGAGTSAARGFPDFGLLTM
SEMVDNAAVMARSVAIPLIADADTGYGNELNVTRTVREYEARGVAAIHIEDQVAPKRCGH
LDGKEVVSREEFVSKIRAAVEARRTKAFIVIARTDARAMLGLDEAIERANAALQAGADMA
FVEATQTIEEVAAVPKRVHGPCLLNVVPGGRTPVFDLREAEAMGYRLAILPGLMLKAAIE
AGDAALAELKATSKAPTVNATVAQTFRRFGADEWDALRTRFNAGATMESR