Protein Info for GFF1679 in Variovorax sp. SCN45

Annotation: Riboflavin synthase eubacterial/eukaryotic (EC 2.5.1.9)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 234 TIGR00187: riboflavin synthase, alpha subunit" amino acids 1 to 201 (201 residues), 174.9 bits, see alignment E=6.1e-56 PF00677: Lum_binding" amino acids 3 to 88 (86 residues), 70.6 bits, see alignment E=4.5e-24 amino acids 101 to 186 (86 residues), 72.8 bits, see alignment E=9.5e-25

Best Hits

Swiss-Prot: 45% identical to RISA_SHIFL: Riboflavin synthase (ribE) from Shigella flexneri

KEGG orthology group: K00793, riboflavin synthase [EC: 2.5.1.9] (inferred from 80% identity to bpe:BP2874)

MetaCyc: 45% identical to riboflavin synthase (Escherichia coli K-12 substr. MG1655)
Riboflavin synthase. [EC: 2.5.1.9]

Predicted SEED Role

"Riboflavin synthase eubacterial/eukaryotic (EC 2.5.1.9)" (EC 2.5.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.9

Use Curated BLAST to search for 2.5.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (234 amino acids)

>GFF1679 Riboflavin synthase eubacterial/eukaryotic (EC 2.5.1.9) (Variovorax sp. SCN45)
MFTGIVQGVARIVAIEDRQGLRSFTIDFPAGFCADLATGASVSVDGVCLTVTQLLSPTSV
TFDVILQSLNVTTLGNYAVGDSVNVERAAKDGAEIGGHPLSGHIDFSAEIMTVQTSDTNR
VLRIGIPEGFRKYVFAKGYIAINGASLTVSEASRAEGWFEVWLIPETRRMTVFENKRAGD
RLNIEIERGTQVVVDTVREAVSESLGKLQPLLEALLREKGLTLEDFVQPPAKLH