Protein Info for GFF1676 in Sphingobium sp. HT1-2

Annotation: DNA polymerase III subunits gamma and tau (EC 2.7.7.7)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 541 TIGR02397: DNA polymerase III, subunit gamma and tau" amino acids 8 to 372 (365 residues), 407.6 bits, see alignment E=2.5e-126 PF13177: DNA_pol3_delta2" amino acids 26 to 187 (162 residues), 132.9 bits, see alignment E=3.9e-42 PF00004: AAA" amino acids 47 to 182 (136 residues), 41.8 bits, see alignment E=5.2e-14 PF22608: DNAX_ATPase_lid" amino acids 194 to 238 (45 residues), 63.5 bits, see alignment 4.3e-21 PF12169: DNA_pol3_gamma3" amino acids 247 to 388 (142 residues), 146.4 bits, see alignment E=1.9e-46 PF12362: DUF3646" amino acids 418 to 529 (112 residues), 93.4 bits, see alignment E=3.9e-30

Best Hits

KEGG orthology group: K02343, DNA polymerase III subunit gamma/tau [EC: 2.7.7.7] (inferred from 81% identity to sjp:SJA_C1-21710)

Predicted SEED Role

"DNA polymerase III subunits gamma and tau (EC 2.7.7.7)" in subsystem DNA-replication (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (541 amino acids)

>GFF1676 DNA polymerase III subunits gamma and tau (EC 2.7.7.7) (Sphingobium sp. HT1-2)
MSDSPQPYRVLARKYRPRNFRELIGQDAMVQTLGNAIRRGRLAHAFLMTGVRGVGKTSTA
RLIAKALNCIGPDGQGGPTIDPCGVCEPCMAIAEGRHIDVVEMDAASHTGVDDVREIIEA
VRYAAVSARYKIYIIDEVHMLSKNAFNALLKTLEEPPAHVKFLFATTEVNKVPVTVLSRC
QRFDLRRIPAEMLAGHFAHVVEAEKVAAEPDALSLIAQAAEGSARDGLSILDQAIAHAEM
GEGEPMVTAAQVREMLGLSDRGSTRRLLGLLLEGDTAALLGGVRDQYALGVEPLSLMRGL
LELVHAVTLVKAGRDIATPGQSDEERGALADWASQIGFGPLHRLWQLMLKGHDEVATAAL
PIEACEMALLRVMHAATMPDPSDLARMLQGGVPTAAPAMLGQASAAAAPAPEPASSLPAT
FEDLIEAFWQKSKGHLAQEMHDCVGIIRYDPPMLEYRGTASLSPDFATRIIPALREVTGV
AWTVTQGEGEAKPTLLANEQRQLADSRAAILETPVVKAAMDAFPDAELVHSDEPEQWSAE
A