Protein Info for GFF1666 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Holliday junction DNA helicase RuvA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 192 PF01330: RuvA_N" amino acids 1 to 62 (62 residues), 83.6 bits, see alignment E=1.7e-27 TIGR00084: Holliday junction DNA helicase RuvA" amino acids 1 to 190 (190 residues), 179.7 bits, see alignment E=2.3e-57 PF14520: HHH_5" amino acids 72 to 129 (58 residues), 61.9 bits, see alignment E=1.3e-20 PF07499: RuvA_C" amino acids 147 to 191 (45 residues), 43.6 bits, see alignment 6.6e-15

Best Hits

Swiss-Prot: 82% identical to RUVA_ACIAC: Holliday junction ATP-dependent DNA helicase RuvA (ruvA) from Acidovorax citrulli (strain AAC00-1)

KEGG orthology group: K03550, holliday junction DNA helicase RuvA (inferred from 82% identity to aav:Aave_4240)

MetaCyc: 47% identical to Holliday junction branch migration complex subunit RuvA (Escherichia coli K-12 substr. MG1655)
3.1.22.4-RXN [EC: 3.1.21.10]

Predicted SEED Role

"Holliday junction DNA helicase RuvA" in subsystem DNA-replication or RuvABC plus a hypothetical

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.21.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (192 amino acids)

>GFF1666 Holliday junction DNA helicase RuvA (Hydrogenophaga sp. GW460-11-11-14-LB1)
MIGKLTGTLLEKNPPQILLDCHGVGYEVDVPMSTFYNLPAAGEKVALLTHFVVREDAQIL
YGFGTAPEREAFRQLIKISGVGPRTALSVLSGMSVADLAQAVSAQESGRIVKVPGIGKKT
AERLLLELKGKLGADLNLPGAGPAQSDVQSDIQQALMALGYSDKDATAALKALPKDVGVS
EGIKLALKALAK