Protein Info for GFF1663 in Methylophilus sp. DMC18

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 589 PF00563: EAL" amino acids 14 to 239 (226 residues), 169.5 bits, see alignment E=1.3e-53 PF00990: GGDEF" amino acids 415 to 500 (86 residues), 55.5 bits, see alignment E=9.4e-19 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 435 to 500 (66 residues), 51.5 bits, see alignment E=4.9e-18

Best Hits

KEGG orthology group: None (inferred from 52% identity to mep:MPQ_1350)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (589 amino acids)

>GFF1663 hypothetical protein (Methylophilus sp. DMC18)
MIYAAPNTEIEVILAQRNLLPLFQPIIDIKQAQVFAHEALIRGPLESKLHAPIALLNAAK
LCGRDIEMESIARDVIFEEYRRANHAMPLFVNFSPDALLHSESDWSLSLAQLENYQLAAS
DIVIEITESSTIRDYAELKEAVTKLKALGFKIALDDLGEGTSSLRLWSELSPHYVKIDKH
FIANIHNDPIKLEFVRGIQKIALESNTLTIAEGIETKEELAVIKDLKIDYAQGYLLGRPF
PKFQATLSEEVSTLLNKNIIRLFADQQTMVSKQATVSGLTSYQVAALPEMTNEEVYDWFH
HDNNLNSIPVTDHKGKPLGLISRYDTIDRFARRYQKELHGKKSCTTFMDTQCLIIQKDMH
ILALSELILQADPKYLLNGFIIVDGERYVGMGSGHALLREVTNMQIHAARYANPLTLLPG
NVPINAHIDKLLERRIAFVACYCDLDHFKPFNDAYGYRRGDEVIQFVGRMLSSMVNHEHD
FVGHIGGDDFVIVFQSEDWERICRDILDTVAKVMPDFYDLKDVQSGGIHIEDRLGNEHFF
PFGSLSIGAVRVSAEFFASHHEVAGAMSHAKKQAKKIVGNSLFTERRKR