Protein Info for GFF1655 in Sphingobium sp. HT1-2

Annotation: Alanyl-tRNA synthetase (EC 6.1.1.7)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 78% identical to SYA_SPHAL: Alanine--tRNA ligase (alaS) from Sphingopyxis alaskensis (strain DSM 13593 / LMG 18877 / RB2256)

KEGG orthology group: K01872, alanyl-tRNA synthetase [EC: 6.1.1.7] (inferred from 75% identity to nar:Saro_1279)

Predicted SEED Role

"Alanyl-tRNA synthetase (EC 6.1.1.7)" (EC 6.1.1.7)

MetaCyc Pathways

tRNA charging (19/21 steps found)
superpathway of chorismate metabolism (41/59 steps found)
shinorine biosynthesis (3/8 steps found)
vanchrobactin biosynthesis (2/8 steps found)
enterobactin biosynthesis (4/11 steps found)
ergotamine biosynthesis (4/12 steps found)
(2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
bacillibactin biosynthesis (3/12 steps found)
superpathway of ergotamine biosynthesis (5/18 steps found)
sulfazecin biosynthesis (1/16 steps found)
phosalacine biosynthesis (5/25 steps found)
phosphinothricin tripeptide biosynthesis (4/25 steps found)
corallopyronin A biosynthesis (2/30 steps found)
colibactin biosynthesis (6/38 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (888 amino acids)

>GFF1655 Alanyl-tRNA synthetase (EC 6.1.1.7) (Sphingobium sp. HT1-2)
MTSTNDIRRSFLDYFGANGHTIVPSAPLVPHNDPTLMFVNAGMVPFKNVFTGLETRPYKT
ATSSQKSVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYFKEQAIHHAWTLLTKEWGL
PAEKLTTTVYHTDDEAFDLWKKIAGLPDHKIIRIPTKDNFWVMGDSGPCGPCSEIFYDHG
DHIWGGPPGSPEEDGDRFVEIWNLVFMQYEQEANEIVSELPKPSIDTGMGLERIAAVMQG
VHNNYDIDTFKALIAESGALTRTATDGEFQASHRVIADHLRSTSFLIADGVLPANEGRGY
VLRRIMRRAMRHAHIIGAKDPLMYRLVSSLVSEMGGAYPELVRAQPLIEETLLREETRFR
KTLENGLKLLDEATADLAEGATLPGETAFKLYDTYGFPYDLTEDALRTQGLSVDRAGFDA
AMAEQKAAARAAWKGSGEKASDEIWYDIAETLGSTEFIGYTSTKGEGEVLAIIKDGARVD
AASAGDKVTIITNQTPFYGESGGQNGDAGTITTLGGLVAEVADTSKPLGRLHAHQAVISA
GSVKVGDTVNLTVDVERRDRIRANHSATHLLHAALRKELGGHVTQKGSMVAADRLRFDFS
HPEALTQAQIARVEADVNAQIRHNDEVMTRLMTPDDAIAAGAMALFGEKYGDEVRVLSMG
AKGLGGDDHSYSVELCGGTHVRALGDIALFKIVSESAVSSGVRRIEALTGEAARLWLVER
DEKLRQTAAALKTTPEEVPARIAALVEQSRKLERELAEAKKALALGGGGSAKPAGPEKVG
SVDFLGQVIDGLDPKELRGIVDGNKKTLGSGVSAVLAVVDGRATIAVGVTDDLTGSFSAV
DLVRAGVAALGGKGGGGRADMAQGGGPDGDKAQAAIDAVKAALASVPA