Protein Info for GFF1638 in Variovorax sp. SCN45

Annotation: Siderophore biosynthesis non-ribosomal peptide synthetase modules

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1093 PF00668: Condensation" amino acids 19 to 465 (447 residues), 308.4 bits, see alignment E=1.7e-95 PF00501: AMP-binding" amino acids 487 to 835 (349 residues), 314.6 bits, see alignment E=1.8e-97 TIGR01733: amino acid adenylation domain" amino acids 506 to 909 (404 residues), 465.5 bits, see alignment E=6.8e-144 PF13193: AMP-binding_C" amino acids 893 to 967 (75 residues), 44.8 bits, see alignment 3.8e-15 PF00550: PP-binding" amino acids 994 to 1057 (64 residues), 57.2 bits, see alignment 3.3e-19

Best Hits

KEGG orthology group: None (inferred from 52% identity to vap:Vapar_3744)

Predicted SEED Role

"Non-ribosomal peptide synthetase modules, pyoverdine??" in subsystem Siderophore Pyoverdine

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1093 amino acids)

>GFF1638 Siderophore biosynthesis non-ribosomal peptide synthetase modules (Variovorax sp. SCN45)
MNQPLMQPTIARTVSEGDAVALSYAQEQLCFLQKLEPGLTAYNLPRVFRLAGRLDVDALE
RAFRALIARHAVLRTRFFEQDGVPMQSVQVDAPFALERIDLSGLNAEAQKAPLDEAVRRA
AGHVFDLGAAPALVACLVKLGDERHVLAVCLHHIVSDAWSNPILARDLAAAYRLALHSAG
PVRLPPLAVQYADYAVWQRASVQGGALERQLAHWNRHLGPEVPALDLPTDHARPSRKSFA
GTALGFDVPPALALAVQKFCRAEKCTPFVALFAAWQVLLARCSGQNDFAIGVPNAGRHHE
EVSELLGFFVTTQVFRARLSPHQSLRQVCRQVRADALAALDNAELPFEVLLASRKDRRDP
ARSPLFQVMFGVQMAGEAVALDFEGVRAELEEFDDAGAKFDLSLDFYIDAQGVRGRLEYN
TDLFEAGTAQQLVARYLRVLETLATDADSVLANLVLTGEDERVHLMRRSENLPHRAYAGP
VHRLIGQQARRTPEAVALVFGDVSLSYAQLEARANRLAHRLIALGVRPDTKVGIASERSI
EMVVGLLAILKAGGAYVPIDPEYPQDRIAYMLEDSGVSLLLTQSHIAPAIPVREGVRTIE
LDLLDLLDLQTGPSVDPDVPLHGEHLAYVIYTSGSTGRPKGAANRHLSLFNRLAWMQDAY
ALHEADTVLQKTPFSFDVSVWEFFWPLMQGARLVVAQPGDHREPGKLVELIRRHGVSTIH
FVPSMLQAFVAHEGIEACKSLKRIVCSGEALPAEAQARVFERLPGAGLYNLYGPTEAAID
VTHWRCRADGRNHVAIGRAIAGTKTYVLDDGLNLTAQGLPGELYLGGIGLARGYLDKSAL
TSERFVADPFSDAGERLYRTGDLVRWREDGELEYLGRIDHQVKIRGFRIELGEIEARLLA
QPGVREAVVVAQQAAGGARLVAYVSAAASQAIDAAQLKQQLSRALPEHMVPGVIVVLQAL
PLNANGKVDRKALPQPETPGAATRVAPTRDIERSICAIWTQVLGLDDVGIHDNFFDIGGH
SLLLVQVQSQIKSQLGFSVPLVDLFRHPTVAAQAHCLSPAYTEETAMQPARDGARRQREA
MLRRKKAAEGMHR