Protein Info for GFF1633 in Xanthobacter sp. DMC5

Annotation: Peptide chain release factor 3

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 535 TIGR00503: peptide chain release factor 3" amino acids 13 to 533 (521 residues), 628.3 bits, see alignment E=9.4e-193 PF00009: GTP_EFTU" amino acids 18 to 274 (257 residues), 166.4 bits, see alignment E=1.4e-52 PF01926: MMR_HSR1" amino acids 22 to 149 (128 residues), 26.8 bits, see alignment E=1.2e-09 TIGR00231: small GTP-binding protein domain" amino acids 22 to 159 (138 residues), 80.9 bits, see alignment E=9e-27 PF22042: EF-G_D2" amino acids 302 to 387 (86 residues), 86.7 bits, see alignment E=2.4e-28 PF03144: GTP_EFTU_D2" amino acids 320 to 385 (66 residues), 41.1 bits, see alignment E=5.1e-14 PF16658: RF3_C" amino acids 393 to 518 (126 residues), 143.7 bits, see alignment E=7.4e-46

Best Hits

Swiss-Prot: 53% identical to RF3_GEOSL: Peptide chain release factor 3 (prfC) from Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)

KEGG orthology group: K02837, peptide chain release factor 3 (inferred from 97% identity to xau:Xaut_2646)

Predicted SEED Role

"Peptide chain release factor 3"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (535 amino acids)

>GFF1633 Peptide chain release factor 3 (Xanthobacter sp. DMC5)
MLDQTPNAALADLPDFARRRTFAIISHPDAGKTTLTEKLLLASGAIRMAGHVKARGERRR
TRSDWMEIEQQRGISVTSSVMTFERDGIVFNLLDTPGHSDFSEDTYRTLSAVDAAVMVID
AAKGIESQTRKLFEVCRLRDIPIFTFVNKVDRESLDPIALMDEVSSTLALDLTPLTWPIG
MGVDFRGLIDIANKRALLATERGGPLVREVPFETPEDLIGLDGVEERILNEAVESLTLAL
GVLPPFDLAAFREGHLSPVYFGSALKEAGVAELLFGLGSVGPSPLPRSAAGDRTVEPAED
LVSGFVFKVQANMDPNHRDRVAFVRLCSGRFKRGMKLFNPREKRNMAIANPIFFFAQERE
TVDEAVAGDIIGIPNHGMLRVGDTLSEGESIKFEGLPDFAPEILRRVLLSDPMKAKQLQK
ALQDMAEEGVVQVLKPVSDPAPIVGVVGTLQLDVLAARLEKEYGVPIRYETTSFVTARWI
VGERTEVERFLELNRSSTARDRDDAPVYLARSQWVLDRAIEEWPKLKFVAVKERA