Protein Info for GFF1629 in Pseudomonas sp. DMC3

Annotation: Gamma-glutamylputrescine synthetase PuuA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00120: Gln-synt_C
  • amino acids 121 to 454 (334 residues), 361.2 bits, E=2.7e-112

Best Hits

Swiss-Prot: 45% identical to PUUA_ECOLI: Gamma-glutamylputrescine synthetase PuuA (puuA) from Escherichia coli (strain K12)

KEGG orthology group: K01915, glutamine synthetase [EC: 6.3.1.2] (inferred from 98% identity to pfo:Pfl01_2122)

MetaCyc: 45% identical to glutamate-putrescine ligase (Escherichia coli K-12 substr. MG1655)
Glutamate--putrescine ligase. [EC: 6.3.1.11]

Predicted SEED Role

"Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11)" (EC 6.3.1.11)

MetaCyc Pathways

• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• L-glutamate and L-glutamine biosynthesis (6/7 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• superpathway of ammonia assimilation (plants) (3/3 steps found)
• superpathway of L-arginine and L-ornithine degradation (10/13 steps found)
• ammonia assimilation cycle I (2/2 steps found)
• ammonia assimilation cycle II (2/2 steps found)
• L-glutamine biosynthesis I (1/1 steps found)
• superpathway of ornithine degradation (6/8 steps found)
• putrescine degradation II (3/4 steps found)
• superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation (8/11 steps found)
• L-aspartate degradation II (aerobic) (2/3 steps found)
• L-aspartate degradation III (anaerobic) (2/3 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Glutamate metabolism
• Nitrogen metabolism
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 6.3.1.2

Use Curated BLAST to search for 6.3.1.11 or 6.3.1.2

Protein Sequence (458 amino acids)

>GFF1629 Gamma-glutamylputrescine synthetase PuuA (Pseudomonas sp. DMC3)
MSVPLRTVQLNEANAFLKKYPEVLYVDLLIADMNGVVRGKRIERTSLHKVYEKGINLPAS
LFALDINGSTVESTGLGLDIGDADRICYPIPGTLSIEPWQKRPTAQLLMTMHEIEGQPFF
ADPREVLANVVRKFDDLGLTICAAFELEFYLIDQDNVNGRPQSPRSPVSGKRPVSTQVYL
IDDLDEYVDCLQDILEGAKEQGIPADAIVKESAPAQFEVNLHHVSDPIKACDYAVLLKRL
VKNIAYDHEMDTTFMAKPYPGQAGNGLHVHISILDKEGNNIFASEDPEQNAALRHAIGGV
LETLPAQMAFLCPNVNSYRRFGAQFYVPNSPSWGIDNRTVAVRVPTGSADAVRIEHRVAG
ADANPYLLMASVLAGIHHGLTNQIEPGAPVEGNSYEQNEQSLPNNLRDALRELDDSEVMA
RYIDPMYIDVFVACKESELAEFENSISDLEYNWYLHTV