Protein Info for PS417_08285 in Pseudomonas simiae WCS417

Annotation: ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 564 transmembrane" amino acids 6 to 28 (23 residues), see Phobius details amino acids 67 to 87 (21 residues), see Phobius details amino acids 136 to 156 (21 residues), see Phobius details amino acids 177 to 197 (21 residues), see Phobius details amino acids 253 to 274 (22 residues), see Phobius details amino acids 286 to 305 (20 residues), see Phobius details amino acids 375 to 400 (26 residues), see Phobius details amino acids 420 to 442 (23 residues), see Phobius details amino acids 487 to 509 (23 residues), see Phobius details amino acids 530 to 554 (25 residues), see Phobius details TIGR00680: K+-transporting ATPase, A subunit" amino acids 5 to 561 (557 residues), 742.3 bits, see alignment E=1.8e-227 PF03814: KdpA" amino acids 11 to 560 (550 residues), 793.5 bits, see alignment E=4.1e-243

Best Hits

Swiss-Prot: 98% identical to KDPA_PSEFS: Potassium-transporting ATPase potassium-binding subunit (kdpA) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K01546, K+-transporting ATPase ATPase A chain [EC: 3.6.3.12] (inferred from 98% identity to pfs:PFLU1676)

MetaCyc: 54% identical to K+ transporting P-type ATPase subunit KdpA (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-2 [EC: 7.2.2.6]

Predicted SEED Role

"Potassium-transporting ATPase A chain (EC 3.6.3.12) (TC 3.A.3.7.1)" in subsystem Potassium homeostasis (EC 3.6.3.12, TC 3.A.3.7.1)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.12

Use Curated BLAST to search for 3.6.3.12 or 7.2.2.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7TXE7 at UniProt or InterPro

Protein Sequence (564 amino acids)

>PS417_08285 ATPase (Pseudomonas simiae WCS417)
MHSYDYWLIIAFFAVVLVPAPFLGRFYYKVMEGQRTWLTPVFGPVERACYRLSGVDEHQE
QSWQKYMLALLAFNLAGFLLLFSILVFQDYLPLNPQKLPGQEWTLAFNTAVSFMTNTNWQ
SYSGEASLSYLSQMAGLTVQNFVSAATGLAVLVALCRGIGRKSAQTLGNFWVDMTRATLY
GLLPLCLVLALFLVWQGVPQTFAHYVDAVTLQGVDQVIPLGPAASQIAIKQLGTNGGGFF
GVNSAHPFEDPTAWANLFEVASIILIPVALVFTFGHYVKDLRQSRAILGCMLALFLIGGA
TSLWSEYQPNPTLNNPAVEQTAPLEGKEARFGTTGTVLWSVTTTAASNGSVNGMQDSLSP
LSGMVALVNMMVGEVIFGGVGAGMYGMLLNVLIAVFLAGLMIGRTPEYLGKKLQAKEVQL
LVVTLLVMPVGVLVLGAIAASLPGPAGAISNPGPHGFSQLLYAYTSASANNGSAFGGFSA
NTPFHNLMLGLGMLIGRFGYILPVLALAGSLAMKKTAPIGQNSFPTHGPLFVTLLTVTIL
LVGGLSFLPTLALGPIAEHMSMGF