Protein Info for GFF1623 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Glucoamylase (EC 3.2.1.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 604 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF19291: TREH_N" amino acids 17 to 166 (150 residues), 55.5 bits, see alignment E=5.1e-19 PF00723: Glyco_hydro_15" amino acids 230 to 593 (364 residues), 160.2 bits, see alignment E=7.9e-51

Best Hits

KEGG orthology group: None (inferred from 78% identity to pna:Pnap_0525)

Predicted SEED Role

"Glucoamylase (EC 3.2.1.3)" in subsystem Maltose and Maltodextrin Utilization or Trehalose Biosynthesis (EC 3.2.1.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (604 amino acids)

>GFF1623 Glucoamylase (EC 3.2.1.3) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MSPTPGFVPPAAASLSLGMVGNCAFSALIDPHGRVVWCCLPRFDGDPVFHALLGADDGGQ
GTFAIEIEGLAEATQRYEHNTAVLVTELWDHQGNGLSITDFAPRFPSRGRYFRPTQLVRR
VRPLKGAPRIRVSVQPRFDWGRSAPTITSGSNHIRYVGEGQTLRLHTDASISHVLARAPF
LLTRECNFMLGPDETLETGIGDTARHFEQETLSYWRNWSRRLAVPLEWQEAVIRAAITLK
LSLYEDTGAIVAAMTTSIPEAPGTQRNWDYRYCWLRDAFFVVRALNSLSEVGTMEDYLRW
LANVVVGAGGGHIQPLHGIGLERELPESICDTLPGYRGMGPVRVGNQAQEHFQHDVYGNI
VLAAAQAFHDERLLHRAGLAEFEQLEAVGERALRIYDQPDAGMWELRTRARVHTSSALMC
WAACDRLGKIAHSLEMPGRAAYWASHAETMRERILRESWNEERQAFAESFGGRDLDASVL
LMVEVGMIDPMDPRFLSTLDALEKHLCDGPFMRRYEAPDDFGKPETAFNICTFWRIDALA
RTGRADEARAIFETMLAARNPLGMLSEDTHATTGEMWGNFPQTYSMVGIINAAVRLSARW
DSVI