Protein Info for GFF1621 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: tRNA nucleotidyltransferase (EC 2.7.7.21) (EC 2.7.7.25)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 420 PF01743: PolyA_pol" amino acids 1 to 131 (131 residues), 51.7 bits, see alignment E=1.7e-17 PF12627: PolyA_pol_RNAbd" amino acids 156 to 218 (63 residues), 43.3 bits, see alignment E=3.8e-15 PF01966: HD" amino acids 237 to 352 (116 residues), 39.9 bits, see alignment E=7.4e-14

Best Hits

Swiss-Prot: 73% identical to CCA_POLNA: Multifunctional CCA protein (cca) from Polaromonas naphthalenivorans (strain CJ2)

KEGG orthology group: K00974, tRNA nucleotidyltransferase (CCA-adding enzyme) [EC: 2.7.7.72 3.1.3.- 3.1.4.-] (inferred from 74% identity to mpt:Mpe_A3298)

Predicted SEED Role

"tRNA nucleotidyltransferase (EC 2.7.7.21) (EC 2.7.7.25)" in subsystem Polyadenylation bacterial or tRNA nucleotidyltransferase (EC 2.7.7.21, EC 2.7.7.25)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.-, 3.1.4.-

Use Curated BLAST to search for 2.7.7.21 or 2.7.7.25 or 2.7.7.72 or 3.1.3.- or 3.1.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (420 amino acids)

>GFF1621 tRNA nucleotidyltransferase (EC 2.7.7.21) (EC 2.7.7.25) (Hydrogenophaga sp. GW460-11-11-14-LB1)
VGGAVRDALMARASETPAVSHADRDWVVVGATPEAMAAEGFLPVGRDFPVFLHPKTKEEY
ALARTERKTAPGYHGFAFHTEAGVTLEDDLARRDLTINAMAVAAEHADDPARAPLIDPHH
GQRDLRERVLRHVTGAFAEDPVRILRLARFAARFPDFSVAPETGALMREMVANGEVDHLV
AERVWQELARGLMEQRPSRMLEVLRECGALQRLLPEVDRLWGVPQRAEYHPEVDTGVHLM
MVLDMSARLAAPLTVRFACLCHDLGKGTTPADVLPRHIGHEERSARLLKGVCERLRVPTD
CREIADVVAREHGNIHRSGDFSAGAVERLLERCDALRKPARFHEVLLACECDARGRLGLE
DEPYPQRERLARALAAARSVDTAVVASQAQADGLSGPRVGERIHRARVTAVAQALGTPGD