Protein Info for PS417_08190 in Pseudomonas simiae WCS417

Annotation: membrane protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 664 transmembrane" amino acids 21 to 41 (21 residues), see Phobius details amino acids 53 to 72 (20 residues), see Phobius details amino acids 84 to 106 (23 residues), see Phobius details amino acids 118 to 136 (19 residues), see Phobius details PF13727: CoA_binding_3" amino acids 82 to 255 (174 residues), 25.4 bits, see alignment E=4.1e-09 PF01370: Epimerase" amino acids 300 to 532 (233 residues), 53.7 bits, see alignment E=6e-18 PF02719: Polysacc_synt_2" amino acids 300 to 607 (308 residues), 414.4 bits, see alignment E=7.4e-128 PF16363: GDP_Man_Dehyd" amino acids 301 to 428 (128 residues), 34.7 bits, see alignment E=4.2e-12 PF01073: 3Beta_HSD" amino acids 302 to 433 (132 residues), 31.6 bits, see alignment E=2.6e-11

Best Hits

KEGG orthology group: None (inferred from 83% identity to pfl:PFL_4305)

MetaCyc: 75% identical to UDP-N-acetylglucosamine 4,6-dehydratase (configuration-retaining) (Pseudomonas aeruginosa)
RXN-13252 [EC: 4.2.1.135]

Predicted SEED Role

"nucleotide sugar epimerase/dehydratase WbpM"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.135

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UCE6 at UniProt or InterPro

Protein Sequence (664 amino acids)

>PS417_08190 membrane protein (Pseudomonas simiae WCS417)
MDSLRARLVGLPRRQKRFLQVATDIVLVWAALWLAFVVRLGFDDLNNPFVVHLWLFLSAP
VIAIPLFIHFGMYRAVMRYFGNDALIAIIKAVSLSALLLALVVYLYSNHQTVVPRSIVFN
YWWLSMVIIGGLRLAMRQYFQGDWFTAAQHVPFTSRDDGLIKVAIYGAGAAGNQLVAALR
MGRVMRPVAFIDDDSGISDRIISGLQVYKPKRIQQMIDETGAEELLLAIPSSTRARRREI
LGYLEGYPLHVRSVPGFMDLASGRVKVDDIQEVDIADLLGRDPVPAQPELLERCIKHRVV
MVSGAGGSIGSELCRQILTLEPTSLLLFDHSEYNLYCILSELEQRVQSESLNVTLLPILG
SVRHQDKLLEVMNTWSVDTVYHAAAYKHVPMVEHNIAEGILNNVIGTLNTAQAALQAGVE
NFVLISTDKAVRPTNVMGSTKRLAELVLQALSKEVAPTLFGDASNISRVNKTRFTMVRFG
NVLGSSGSVIPLFHKQIQAGGPLTVTHPKITRYFMTIPEAAQLVIQAGSMGQGGDVFVLD
MGEPVKIVELAEKMIHLSGLSIRSDTNPHGDILINFSGLRPGEKLYEELLIGDNVAATRH
PMIMRANEELLGWEVLKARLHGLLTAVEAGDFALVRQLLKNTVSGYSPEGEIVDWMHIQR
HREV