Protein Info for GFF1609 in Variovorax sp. SCN45

Annotation: 2-haloalkanoic acid dehalogenase (EC 3.8.1.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 241 TIGR01428: haloacid dehalogenase, type II" amino acids 6 to 205 (200 residues), 150.2 bits, see alignment E=1.1e-47 PF00702: Hydrolase" amino acids 6 to 177 (172 residues), 45.9 bits, see alignment E=9.1e-16 TIGR01493: HAD hydrolase, family IA, variant 2" amino acids 8 to 188 (181 residues), 41.5 bits, see alignment E=2.4e-14 PF13419: HAD_2" amino acids 11 to 174 (164 residues), 32.4 bits, see alignment E=1e-11

Best Hits

KEGG orthology group: K01560, 2-haloacid dehalogenase [EC: 3.8.1.2] (inferred from 78% identity to reu:Reut_B5659)

MetaCyc: 59% identical to azetidine-2-carboxylate hydrolase (Pseudomonas sp. A2C)
3.5.2.-

Predicted SEED Role

"2-haloalkanoic acid dehalogenase"

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.8.1.2

Use Curated BLAST to search for 3.8.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (241 amino acids)

>GFF1609 2-haloalkanoic acid dehalogenase (EC 3.8.1.2) (Variovorax sp. SCN45)
MKLSDFKALTFDCYGTLIDWESGIYNALEPLRAKAGNDKTRDQVLENYARHESAQEEETP
AMLYPQLLALVYKRLAGEWGITVGDEEANIFGASVPDWPEFADSVEALAYLKKHYKLVIL
SNVDRLSFRSSNARLQVAFDAIYTAQDIGSYKPSPRNFEYMLRRLESDFGLQKKDVLHTA
QSLFHDHAPANGFGLASAWIDRRHASKGWGATMPPPGTPKLDFTFESMIALARAHQAEVG
A