Protein Info for GFF1600 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01173: UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase
  • amino acids 8 to 470 (463 residues), 579.9 bits, E=1.8e-178
• PF12804: NTP_transf_3
  • amino acids 9 to 134 (126 residues), 65.8 bits, E=7.9e-22
• PF00483: NTP_transferase
  • amino acids 10 to 220 (211 residues), 41.5 bits, E=1.9e-14
• PF00132: Hexapep
  • amino acids 280 to 311 (32 residues), 30 bits, see alignment (E = 4.7e-11)
  • amino acids 332 to 367 (36 residues), 27.3 bits, 3.2e-10

Best Hits

Swiss-Prot: 81% identical to GLMU_POLNA: Bifunctional protein GlmU (glmU) from Polaromonas naphthalenivorans (strain CJ2)

KEGG orthology group: K04042, bifunctional UDP-N-acetylglucosamine pyrophosphorylase / Glucosamine-1-phosphate N-acetyltransferase [EC: 2.3.1.157 2.7.7.23] (inferred from 81% identity to pna:Pnap_0533)

MetaCyc: 50% identical to fused N-acetylglucosamine-1-phosphate uridyltransferase and glucosamine-1-phosphate acetyltransferase (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylglucosamine diphosphorylase. [EC: 2.7.7.23]; Glucosamine-1-phosphate N-acetyltransferase. [EC: 2.7.7.23, 2.3.1.157]

Predicted SEED Role

"N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)" in subsystem Peptidoglycan Biosynthesis or Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.3.1.157, EC 2.7.7.23)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (4/7 steps found)
• chitin biosynthesis (5/9 steps found)
• peptidoglycan recycling I (8/14 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (6/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.157 or 2.7.7.23

Protein Sequence (472 amino acids)

>GFF1600 N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157) (Hydrogenophaga sp. GW460-11-11-14-LB1)
VFMSFPVDVVVMAAGKGTRMKSLRPKVLHRLGGRALAQHVIDCAARLSARSVVVITGHGA
EQVEAGLTTPVAAGDSAAPRLRFVRQEPQLGTGHAVQQAAPVLPDDGVTLVLSGDVPLTR
PETLQALLDLCAGERLALLTLDMPDPTGYGRIVRSGDASVQAIVEHKDASEAQRAITEIY
SGIMAVPTRLLRGWLARLDNRNAQSEYYLTDVVKFAVADGLPVVAHRIRDAVQVAGVNSP
VQLAELERAYQRRLAETLMEQGVRLADPARLDVRGELLCGQDVEIDVNCVFEGRVKLGDG
VRIGANCVIANAAIDAGAVIHAFTHIDGEKLGVSVGRGALVGPFARLRPGAQLGEEVHIG
NFVEVKNSTLAAGAKANHLAYLGDATVGERVNYGAGSITANYDGANKHRTVIEADVHVGS
NCVLVAPITIGAGGTVGGGSTLTKSTPPGALAVARGKQMVLDGWQRPTKKKS