Protein Info for GFF159 in Pseudomonas sp. DMC3

Annotation: Ubiquinone biosynthesis O-methyltransferase, mitochondrial

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 331 PF12840: HTH_20" amino acids 24 to 71 (48 residues), 38.3 bits, see alignment 4.7e-13 PF01022: HTH_5" amino acids 25 to 70 (46 residues), 33.8 bits, see alignment 1.1e-11 PF13489: Methyltransf_23" amino acids 150 to 299 (150 residues), 63.9 bits, see alignment E=7.1e-21 PF00891: Methyltransf_2" amino acids 156 to 269 (114 residues), 25.1 bits, see alignment E=4.7e-09 PF13847: Methyltransf_31" amino acids 165 to 276 (112 residues), 63.8 bits, see alignment E=6.6e-21 PF01209: Ubie_methyltran" amino acids 166 to 270 (105 residues), 27.6 bits, see alignment E=8.2e-10 PF08242: Methyltransf_12" amino acids 170 to 262 (93 residues), 58.1 bits, see alignment E=5.5e-19 PF13649: Methyltransf_25" amino acids 170 to 260 (91 residues), 60.1 bits, see alignment E=1.2e-19 PF08241: Methyltransf_11" amino acids 171 to 263 (93 residues), 65.3 bits, see alignment E=3e-21

Best Hits

KEGG orthology group: K03892, ArsR family transcriptional regulator (inferred from 94% identity to pfo:Pfl01_5268)

Predicted SEED Role

"Transcriptional regulator, ArsR family / Methyltransferase fusion"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (331 amino acids)

>GFF159 Ubiquinone biosynthesis O-methyltransferase, mitochondrial (Pseudomonas sp. DMC3)
MNLRVPSIRHDDCDELAALCKAGGDPLRLNVLRALANDSFGVLELAQIFGIGQSGMSHHL
KVLAQADLVATRREGNAIFYRRALPHSELLGGKLHAALLEEVDNLDLPDEVQARIEQVHG
QRAAASRDFFARVAEKFRAQQDLIAGLPQYRDSVLALLDKLNFDGAATAIEVGPGDGAFL
PELARRFGTVTALDNSPAMLELARQVCEREQLANVSLQLADALNGTSLQADCVVLNMVLH
HFAAPADALRHMADLLQPGGSLLVTELCSHNQSWAREACGDLWLGFEQDDLARWATAAGL
VPGESLYVGLRNGFQIQVRHFQRPAGDTHHR