Protein Info for GFF1576 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Glutaminase (EC 3.5.1.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03814: glutaminase A
  • amino acids 7 to 303 (297 residues), 401.3 bits, E=1.1e-124
• PF04960: Glutaminase
  • amino acids 22 to 303 (282 residues), 374.1 bits, E=2.2e-116

Best Hits

Swiss-Prot: 55% identical to GLSA_AGRFC: Glutaminase (glsA) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K01425, glutaminase [EC: 3.5.1.2] (inferred from 77% identity to vpe:Varpa_0145)

Predicted SEED Role

"Glutaminase (EC 3.5.1.2)" in subsystem Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 3.5.1.2)

MetaCyc Pathways

• L-glutamate and L-glutamine biosynthesis (7/7 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• D-Glutamine and D-glutamate metabolism
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.1.2

Protein Sequence (303 amino acids)

>GFF1576 Glutaminase (EC 3.5.1.2) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MQFQPVLDDIVRTLRPRLGMEGRVADYIPALARVPAAQFGIALRTCDGQEAQAGDSATPF
SIQSISKLFALTLGMRALGESLWERIGREPSGNPFNSLVQLEAEQGTPRNPFINAGAIAV
TDRLVSQGDAKAAMLALVSSLCDEPVRFDEEVAASEAATGFRNIALGNFMKSFGKIDNDV
ATVLDAYFHHCAVRMTCLQLARATGYLCRDGQHPFTGERLLTERQTRRINALMLTCGTYD
AAGEFAFRIGLPCKSGVGGGIVAVVPDQLSLCVWSPALDATGNSLLGMQALELFTARTGL
SVF