Protein Info for PS417_08005 in Pseudomonas simiae WCS417

Annotation: trans-2-enoyl-CoA reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 397 PF12242: Eno-Rase_NADH_b" amino acids 2 to 79 (78 residues), 129.3 bits, see alignment E=5.5e-42 PF12241: Enoyl_reductase" amino acids 82 to 316 (235 residues), 358.3 bits, see alignment E=2.5e-111 PF07055: Eno-Rase_FAD_bd" amino acids 322 to 385 (64 residues), 95 bits, see alignment E=4.2e-31

Best Hits

Swiss-Prot: 100% identical to FABV_PSEFS: Enoyl-[acyl-carrier-protein] reductase [NADH] (fabV) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU1621)

Predicted SEED Role

"Short-chain alcohol dehydrogenase family"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U913 at UniProt or InterPro

Protein Sequence (397 amino acids)

>PS417_08005 trans-2-enoyl-CoA reductase (Pseudomonas simiae WCS417)
MIIKPRVRGFICVTAHPVGCEANVKEQIDYVTQHGVIEGGPKKVLVLGASTGYGLAARIS
AAFGCGADTLGVFFEKEGEEGKLSSAGWYNSAAFEKFAVEKGLYAKSINGDAFSDEIKRL
TIETIKKDLGKIDLVVYSLAAPRRTDPQGVVHNSTLKPIGKAVTLRGINTDKGVVVDTTL
EPATQEEIDGTVKVMGGEDWQLWIDALRDADVLAEGAKTTAFTYLGEKLTQDIYWNGSIG
EAKKDLDKKVLTLRDNLAALKGDARVSVLKAVVTQASSAIPIMPLYLSLLFKVMKEQGTH
EGCIEQVYGLFKDSLYGSQPKLDADGRLRADLAELEPKVQDAVAALWNQVTDDNVNEISD
FAGYKAEFLRLFGFEIDGVDYDADVNPTVKINGLISA