Protein Info for GFF1570 in Variovorax sp. SCN45
Annotation: Muconate cycloisomerase (EC 5.5.1.1)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: None (inferred from 84% identity to del:DelCs14_4251)Predicted SEED Role
"Muconate cycloisomerase (EC 5.5.1.1)" in subsystem Catechol branch of beta-ketoadipate pathway or Muconate lactonizing enzyme family (EC 5.5.1.1)
MetaCyc Pathways
- aromatic compounds degradation via β-ketoadipate (8/9 steps found)
- superpathway of salicylate degradation (6/7 steps found)
- catechol degradation III (ortho-cleavage pathway) (5/6 steps found)
- catechol degradation to β-ketoadipate (3/4 steps found)
- mandelate degradation to acetyl-CoA (9/18 steps found)
- superpathway of aromatic compound degradation via 3-oxoadipate (21/35 steps found)
KEGG Metabolic Maps
- 1,4-Dichlorobenzene degradation
- Benzoate degradation via hydroxylation
- Fluorobenzoate degradation
- Toluene and xylene degradation
Isozymes
Compare fitness of predicted isozymes for: 5.5.1.1
Use Curated BLAST to search for 5.5.1.1
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (374 amino acids)
>GFF1570 Muconate cycloisomerase (EC 5.5.1.1) (Variovorax sp. SCN45) VPVIQSIDVCVARVPLDRVTSFSNRTVHERHYGLVKVRSADGVEGIGFCYVGSAAGELFR VAVEQLLAPVLLGQDSLAVEGLWQRMYQESLLQGRMGTVMRALSALDIALWDLNARTVGL PLHKYLGAVELDTVPAYASGGYYLDGKTAEMVGEEMASYVALGFNAVKMKTGRWDPKGEE SRVRAAREAIGPDVQLMLDCNNGWVDTVQAMEYMRRIEPYHPYFIEEPFGPDDIESHARL AKLTCVPVATAEIGYGRWYHKQLLDMAGAAILQTDAAVCGGITEWRRIAAMAAGYGVQMC PHWFHDLHAPLVAATPNARYVEFFWDDQVLNFRRLVDRQLSHQRGRVVLHQTPGLGFEFS AKEVEKYGRWSRCA