Protein Info for GFF1545 in Variovorax sp. SCN45

Annotation: Pyruvate dehydrogenase (quinone) (EC 1.2.5.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 572 PF02776: TPP_enzyme_N" amino acids 5 to 119 (115 residues), 108.4 bits, see alignment E=3e-35 PF00205: TPP_enzyme_M" amino acids 191 to 318 (128 residues), 111.3 bits, see alignment E=4.7e-36 PF02775: TPP_enzyme_C" amino acids 379 to 525 (147 residues), 125.7 bits, see alignment E=2e-40

Best Hits

KEGG orthology group: K00156, pyruvate dehydrogenase (quinone) [EC: 1.2.5.1] (inferred from 74% identity to pau:PA14_69925)

Predicted SEED Role

"Pyruvate oxidase [ubiquinone, cytochrome] (EC 1.2.2.2)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 1.2.2.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.2.2 or 1.2.5.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (572 amino acids)

>GFF1545 Pyruvate dehydrogenase (quinone) (EC 1.2.5.1) (Variovorax sp. SCN45)
MAQRKVAELVVETLQHAGVKHCYGIVGDTLNHVTDAIHRSDIEWVHVRHEEAAAFAAGAE
SYLTGELTACAGSCGPGGLHFINGVFEANRNRAPVVLIASQVVTTELGMEFPQEVDFKSL
YANCTVFCEQVYSAQQAQRLTALAAQAALSRKGVAVIILPSDIAQTEVKDGLTYAVHRAR
PVLRPNDAELSQLAALLGKGRKIAIYAGHGCEGAHEMLLALGQCLKAPIAHTSRAKDFVE
YDNPFNMGMTGIFGVESGYRALQECDTLLLLGADFAWSQFYPAKATILQIDIDGSHLGRR
HPVALGLVGDVANTIEALIPLLEERTDRSFLDDCLRHRKEAVDTLRRDERPGKDGRIHPQ
HLTRIIDELAADDAIFTADGGSPMVWSLRHLTMNGRRRTLVSLLHGTMANAMPQALGAQK
AYPGRQVVSLSGDGGIAMLLGDLMTAVQEKLPIKVVVYNNASLNFVELEQKVEGLLDNYT
DLLNPDFARLAEVIGFYGQKVERAEQLPGAVRAFLAHPGPALLDVTVNRTELVMPPKVEF
SQVAGTMLYSAKAVLSGRSSDVVDLLKNNFTQ