Protein Info for PS417_07680 in Pseudomonas simiae WCS417

Annotation: ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 448 signal peptide" amino acids 1 to 37 (37 residues), see Phobius details transmembrane" amino acids 155 to 175 (21 residues), see Phobius details TIGR01386: heavy metal sensor kinase" amino acids 4 to 445 (442 residues), 488.9 bits, see alignment E=8.6e-151 PF21085: CusS" amino acids 5 to 103 (99 residues), 27.2 bits, see alignment E=7.7e-10 PF00672: HAMP" amino acids 173 to 225 (53 residues), 46.5 bits, see alignment 7.2e-16 PF00512: HisKA" amino acids 231 to 296 (66 residues), 57.5 bits, see alignment E=2.2e-19 PF02518: HATPase_c" amino acids 339 to 446 (108 residues), 94.7 bits, see alignment E=9.6e-31

Best Hits

KEGG orthology group: K07644, two-component system, OmpR family, heavy metal sensor histidine kinase CusS [EC: 2.7.13.3] (inferred from 91% identity to pfs:PFLU1575)

Predicted SEED Role

"Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-)" in subsystem Potassium homeostasis (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UC48 at UniProt or InterPro

Protein Sequence (448 amino acids)

>PS417_07680 ATPase (Pseudomonas simiae WCS417)
MIKRLSLASRLALLFAACTAVVSLIAGVLFNHASEAHFIELDQQQLDSQLVALRGTLQGV
DSPGTFARREAQLRAELNHQPELRVRISAAGQRWLDDAPGVNLPDAPGLHSLQNAGTDYR
VYNAPLTPGQPDSPLLTLMLDITHHQHFLQRMQHLIWLTVGLSALATALLGAWAARSGLQ
PLRRMGEVAASVSAHSLTQRLPQQHMPVELAELAQAFNAMLGRLDDGFQRLSAFSADIAH
ELRTPLSNLLTQTQVILTQPRPLEDYREALHSNLEELQWMAQLVNDMLYLAKADHGLLMP
KRVPLALADEVDALLEFFALLAEDAQVSLIREGTAHTLGDRGMLRRALSNLLDNALRFTP
AGGEVRVRMTEGVTLTVENTGEGIPPELLPKLFDRFYRADPARREGSSEHAGLGLAITQS
IVRAHGGRIFCESEVGLTRFVIELPKGD