Protein Info for GFF1502 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 721 TIGR03591: polyribonucleotide nucleotidyltransferase" amino acids 20 to 701 (682 residues), 1086.9 bits, see alignment E=0 PF01138: RNase_PH" amino acids 25 to 154 (130 residues), 98.9 bits, see alignment E=7.6e-32 amino acids 334 to 466 (133 residues), 94.7 bits, see alignment E=1.6e-30 PF03725: RNase_PH_C" amino acids 157 to 220 (64 residues), 57.2 bits, see alignment E=3.2e-19 amino acids 470 to 537 (68 residues), 25.1 bits, see alignment E=3.4e-09 PF03726: PNPase" amino acids 252 to 330 (79 residues), 95 bits, see alignment E=7.8e-31 PF00013: KH_1" amino acids 566 to 624 (59 residues), 38.8 bits, see alignment 1.6e-13 PF00575: S1" amino acids 629 to 700 (72 residues), 81.2 bits, see alignment E=1.5e-26

Best Hits

Swiss-Prot: 98% identical to PNP_CITK8: Polyribonucleotide nucleotidyltransferase (pnp) from Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)

KEGG orthology group: K00962, polyribonucleotide nucleotidyltransferase [EC: 2.7.7.8] (inferred from 98% identity to cro:ROD_46681)

MetaCyc: 97% identical to polynucleotide phosphorylase (Escherichia coli K-12 substr. MG1655)
Exoribonuclease II. [EC: 3.1.13.1]; Polyribonucleotide nucleotidyltransferase. [EC: 3.1.13.1, 2.7.7.8]

Predicted SEED Role

"Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8)" in subsystem Bacterial RNA-metabolizing Zn-dependent hydrolases or Polyadenylation bacterial (EC 2.7.7.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.13.1

Use Curated BLAST to search for 2.7.7.8 or 3.1.13.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (721 amino acids)

>GFF1502 Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MPSKIGKDIILLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTVVGQKKA
KPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPVRPLFPEGFVNE
VQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQYVLNPTQDEL
KESKLDLVVAGTEAAVLMVESEAELLSEDTMLGAVVFGHEQQQVVIQAINDLVKEAGKPR
WDWQPEAVNDALNARVAALAESRLSDAYRITDKQERYAQVDVIKSETIEQLIAEDETLDA
NELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVLPRTHGSALFTRGETQA
LVTATLGTARDAQVLDELMGERTDSFLFHYNFPPYSVGETGMVGSPKRREIGHGRLAKRG
VLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLALMDAGVPIKAAVAGIAMGLVK
EGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMDIKIEGITKEIMQVALNQAKGAR
LHILGVMEQAINAPRGDISEFAPRIHTIKISTDKIKDVIGKGGSVIRALTEETGTTIEIE
DDGTVKIAATDGEKAKYAIRRIEEITAEIEVGRIYNGKVTRIVDFGAFVAIGGGKEGLVH
ISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQGRVRLSIKEATEQSQPAAAPEAPASEQA
E